{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:09:15Z","timestamp":1761059355171},"reference-count":0,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[1987,9,1]],"date-time":"1987-09-01T00:00:00Z","timestamp":557452800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1987,9]]},"abstract":"<jats:p>Methicillin- and cephem-resistant Staphylococcus aureus (137 strains) for which the cefazolin MICs are at least 25 micrograms\/ml could be classified into low-resistance (83% of strains) and high-resistance (the remaining 17%) groups by the MIC of flomoxef (6315-S), a 1-oxacephalosporin. The MICs were less than 6.3 micrograms\/ml and more than 12.5 micrograms\/ml in the low- and high-resistance groups, respectively. All strains produced penicillin-binding protein 2' (PBP 2'), which has been associated with methicillin resistance and which has very low affinity for beta-lactam antibiotics. Production of PBP 2' was regulated differently in low- and high-resistance strains. With penicillinase-producing strains of the low-resistance group, cefazolin, cefamandole, and cefmetazole induced PBP 2' production about 5-fold, while flomoxef induced production 2.4-fold or less. In contrast, penicillinase-negative variants of low-resistance strains produced PBP 2' constitutively in large amounts and induction did not occur. With high-resistance strains, flomoxef induced PBP 2' to an extent similar to that of cefazolin in both penicillinase-producing and -negative strains, except for one strain in which the induction did not occur. The amount of PBP 2' induced by beta-lactam antibiotics in penicillinase-producing strains of the low-resistance group correlated well with resistance to each antibiotic. Large amounts of PBP 2' in penicillinase-negative variants of the low-resistance group did not raise the MICs of beta-lactam compounds, although these strains were more resistant when challenged with flomoxef for 2 h. Different regulation of PBP 2' production was demonstrated in the high- and low-resistance groups, and factor(s) other than PBP 2' were suggested to be involved in the methicillin resistance of high-resistance strains.<\/jats:p>","DOI":"10.1128\/aac.31.9.1307","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:04:52Z","timestamp":1340841892000},"page":"1307-1311","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["Production of low-affinity penicillin-binding protein by low- and high-resistance groups of methicillin-resistant Staphylococcus aureus"],"prefix":"10.1128","volume":"31","author":[{"given":"K","family":"Murakami","sequence":"first","affiliation":[{"name":"Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Nomura","sequence":"additional","affiliation":[{"name":"Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Doi","sequence":"additional","affiliation":[{"name":"Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.31.9.1307","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.31.9.1307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T15:00:14Z","timestamp":1645455614000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.31.9.1307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,9]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1987,9]]}},"alternative-id":["10.1128\/AAC.31.9.1307"],"URL":"https:\/\/doi.org\/10.1128\/aac.31.9.1307","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,9]]},"assertion":[{"value":"1987-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}