{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T16:26:37Z","timestamp":1777566397545,"version":"3.51.4"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[1987,9,1]],"date-time":"1987-09-01T00:00:00Z","timestamp":557452800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1987,9]]},"abstract":"<jats:p>Kinetoplastid hemoflagellates are sensitive to growth inhibition by various purine analogs. In this study the activities of 9-deazainosine (9-DINO), formycin B, and sinefungin were compared in experimental murine Trypanosoma brucei subsp. brucei infections, both singly and in combination with the ornithine decarboxylase inhibitor DL-alpha-difluoromethylornithine (DFMO, eflornithine). Used singly, all of the purine analogs were able to suppress an acute T. brucei subsp. brucei infection. 9-DINO and formycin B were the most active. None of the purine analogs was curative when used singly against a strain causing chronic central nervous system infection. 9-DINO was highly effective when used in combination with DFMO in curing this central nervous system infection and another more stringent experimental infection. Neither sinefungin nor formycin B was active in combination with DFMO in curing the central nervous system experimental infection. 9-DINO was metabolized to phosphorylated derivatives of 9-deazaadenosine and 9-deazaguanosine by bloodstream trypomastigotes, but not by murine erythrocyte suspensions or kidney or liver homogenates--a potential rationale for the selectivity of the analog. These studies indicate that 9-DINO is a potent, nontoxic purine analog which, in combination with DFMO, is capable of late-stage cures of African trypanosomiasis.<\/jats:p>","DOI":"10.1128\/aac.31.9.1406","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:04:52Z","timestamp":1340841892000},"page":"1406-1413","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Synergism between 9-deazainosine and DL-alpha-difluoromethylornithine in treatment of experimental African trypanosomiasis"],"prefix":"10.1128","volume":"31","author":[{"given":"C J","family":"Bacchi","sequence":"first","affiliation":[{"name":"Department of Biology, Pace University, New York, New York 10038."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R L","family":"Berens","sequence":"additional","affiliation":[{"name":"Department of Biology, Pace University, New York, New York 10038."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H C","family":"Nathan","sequence":"additional","affiliation":[{"name":"Department of Biology, Pace University, New York, New York 10038."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R S","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Biology, Pace University, New York, New York 10038."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I A","family":"Elegbe","sequence":"additional","affiliation":[{"name":"Department of Biology, Pace University, New York, New York 10038."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K V","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Biology, Pace University, New York, New York 10038."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P P","family":"McCann","sequence":"additional","affiliation":[{"name":"Department of Biology, Pace University, New York, New York 10038."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J J","family":"Marr","sequence":"additional","affiliation":[{"name":"Department of Biology, Pace University, New York, New York 10038."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.31.9.1406","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.31.9.1406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T15:00:41Z","timestamp":1645455641000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.31.9.1406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,9]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1987,9]]}},"alternative-id":["10.1128\/AAC.31.9.1406"],"URL":"https:\/\/doi.org\/10.1128\/aac.31.9.1406","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,9]]},"assertion":[{"value":"1987-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}