{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:43:20Z","timestamp":1760204600493},"reference-count":0,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[1988,11,1]],"date-time":"1988-11-01T00:00:00Z","timestamp":594345600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1988,11]]},"abstract":"<jats:p>Infections caused by strains of Klebsiella pneumoniae resistant to broad-spectrum cephalosporins have been observed recently in hospitals in Clermont-Ferrand, France. beta-Lactam resistance resulted primarily from the plasmid-mediated, expanded-spectrum CTX-1 beta-lactamase. Furthermore, since 1987 some K. pneumoniae isolates more resistant to ceftazidime than to other cephalosporins have been observed. This new resistance phenotype was the result of the production of ceftazidimase CAZ-1 and, more recently, CAZ-2. As in CTX-1-producing strains, resistance to beta-lactams resulting from CAZ-2 was associated with resistance to aminoglycosides except gentamicin, sulfonamide, and tetracycline and was transferable to Escherichia coli by conjugation. Agarose gel electrophoresis of plasmid DNA from wild-type strains and transconjugants indicated that CAZ-2 production was mediated by a plasmid of 85 kilobases highly related to plasmid pCFF04 coding for CTX-1 beta-lactamase. The isoelectric point, close to 6.0, of this novel enzyme differed from those of CTX-1 and CAZ-1. Like CAZ-1, the CAZ-2 enzyme efficiently hydrolyzed ceftazidime and aztreonam, but as with CTX-1, cefotaxime gave the maximal reaction rate. For each expanded-spectrum beta-lactamase, the activity of broad-spectrum cephalosporins was restored by clavulanic acid or sulbactam.<\/jats:p>","DOI":"10.1128\/aac.32.11.1660","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:04:52Z","timestamp":1340841892000},"page":"1660-1665","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Comparative study of a novel plasmid-mediated beta-lactamase, CAZ-2, and the CTX-1 and CAZ-1 enzymes conferring resistance to broad-spectrum cephalosporins"],"prefix":"10.1128","volume":"32","author":[{"given":"C M","family":"Chanal","sequence":"first","affiliation":[{"name":"Service de Bact\u00e9riologie, Facult\u00e9s de M\u00e9decine et Pharmacie, Clermont-Ferrand, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D L","family":"Sirot","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Facult\u00e9s de M\u00e9decine et Pharmacie, Clermont-Ferrand, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Labia","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Facult\u00e9s de M\u00e9decine et Pharmacie, Clermont-Ferrand, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Petit","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Facult\u00e9s de M\u00e9decine et Pharmacie, Clermont-Ferrand, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Morand","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Facult\u00e9s de M\u00e9decine et Pharmacie, Clermont-Ferrand, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J L","family":"Sirot","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Facult\u00e9s de M\u00e9decine et Pharmacie, Clermont-Ferrand, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R A","family":"Cluzel","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, Facult\u00e9s de M\u00e9decine et Pharmacie, Clermont-Ferrand, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.32.11.1660","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.32.11.1660","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T15:01:46Z","timestamp":1645455706000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.32.11.1660"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1988,11]]}},"alternative-id":["10.1128\/AAC.32.11.1660"],"URL":"https:\/\/doi.org\/10.1128\/aac.32.11.1660","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,11]]},"assertion":[{"value":"1988-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}