{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:26:54Z","timestamp":1791077214358,"version":"4.1.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1988,7,1]],"date-time":"1988-07-01T00:00:00Z","timestamp":583718400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1988,7]]},"abstract":"<jats:p>Cytolytic activity of liposomes consisting of stearylamine and phosphatidylcholine (SA\/PC-liposomes) was examined in vitro against the bloodstream forms of Trypanosoma brucei gambiense. More than 99% of the cells (2 X 10(6)\/ml) were killed within 30 min by treatment with 15 mol% SA\/PC-liposomes (100 microM total lipids). As few as 1.2 X 10(12) liposomes per ml (equivalent to 2 nM liposome) showed trypanocidal activity. Fluorescence microscopy of cells treated with the dansylated SA\/PC-liposomes suggested that the liposomes bound to and accumulated on the cell surface, eventually damaging the plasma membrane. SA\/PC-liposomes showed no significant hemolysis when incubated with human and mouse erythrocytes under conditions that killed greater than 99.9% of the T. b. gambiense trypomastigotes. Human leukocytes were also shown to be less susceptible to SA\/PC-liposomes than T. b. gambiense. These results may point to a new direction in strategy for therapy of African trypanosomiasis.<\/jats:p>","DOI":"10.1128\/aac.32.7.966","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:04:52Z","timestamp":1340841892000},"page":"966-970","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["In vitro lysis of the bloodstream forms of Trypanosoma brucei gambiense by stearylamine-bearing liposomes"],"prefix":"10.1128","volume":"32","author":[{"given":"H","family":"Tachibana","sequence":"first","affiliation":[{"name":"Department of Parasitology, School of Medicine, Tokai University, Kanagawa, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Yoshihara","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Medicine, Tokai University, Kanagawa, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Kaneda","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Medicine, Tokai University, Kanagawa, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Nakae","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Medicine, Tokai University, Kanagawa, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.32.7.966","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.32.7.966","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T15:04:08Z","timestamp":1645455848000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.32.7.966"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,7]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1988,7]]}},"alternative-id":["10.1128\/AAC.32.7.966"],"URL":"https:\/\/doi.org\/10.1128\/aac.32.7.966","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,7]]},"assertion":[{"value":"1988-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}