{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:55:43Z","timestamp":1791341743014,"version":"4.3.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1991,5,1]],"date-time":"1991-05-01T00:00:00Z","timestamp":673056000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1991,5]]},"abstract":"<jats:p>The tetracycline (Tet) determinants, which encode resistance either to tetracyclines without minocycline (Tcr) or to tetracyclines including minocycline (Tcr-Mnr), of 30 wild-type clinical isolates of Enterococcus faecium were identified and localized. The Tet determinants were transferred by conjugation into a plasmid-free Enterococcus faecalis recipient at frequencies of 10(-6) to 10(-9) transconjugants per donor, as follows: Tcr, 6 strains; Tcr-Mnr, 14 strains; both Tcr and Tcr-Mnr, 6 strains; no detectable transfer, 4 strains. Classes L (Tcr phenotype) and M and O (Tcr-Mnr phenotype) of the Tet determinants were identified by DNA-DNA hybridization experiments. The Tet L determinant was plasmid-borne in 18 strains and was chromosomal in 2 strains. Tet M was chromosomal in 27 strains and plasmid-borne (pIP1534) in 1 strain; pIP1534 also carried Tet L. Tet M was located on Tn916-like elements in 22 strains and on a Tn916-modified element in 1 strain. Tet O was detected in only one strain in which it was plasmid-borne. Both Tet L and Tet M determinants were carried by 19 strains. One strain carried, in addition to chromosomal nonconjugative Tet L and Tet M determinants, a conjugative Tcr-Mnr marker which did not correspond to any Tet determinant tested in this study. These results attest to the genetic complexity of tetracycline resistance in E. faecium strains.<\/jats:p>","DOI":"10.1128\/aac.35.5.808","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:05:50Z","timestamp":1340841950000},"page":"808-812","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Tetracycline resistance heterogeneity in Enterococcus faecium"],"prefix":"10.1128","volume":"35","author":[{"given":"F","family":"Bentorcha","sequence":"first","affiliation":[{"name":"Laboratoire des Staphylocoques et des Streptocoques, Institut Pasteur, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"De Cesp\u00e9d\u00e8s","sequence":"additional","affiliation":[{"name":"Laboratoire des Staphylocoques et des Streptocoques, Institut Pasteur, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Horaud","sequence":"additional","affiliation":[{"name":"Laboratoire des Staphylocoques et des Streptocoques, Institut Pasteur, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.35.5.808","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.35.5.808","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T15:25:17Z","timestamp":1645457117000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.35.5.808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1991,5]]}},"alternative-id":["10.1128\/AAC.35.5.808"],"URL":"https:\/\/doi.org\/10.1128\/aac.35.5.808","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,5]]},"assertion":[{"value":"1991-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}