{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:03:23Z","timestamp":1761581003256},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1992,4,1]],"date-time":"1992-04-01T00:00:00Z","timestamp":702086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1992,4]]},"abstract":"<jats:p>Unlike other gram-negative enteric bacteria, Vibrio cholerae cells were equally susceptible to penicillin and ampicillin and in general more susceptible than Escherichia coli to most of the beta-lactam antibiotics. The turbidity of penicillin-treated cultures contained to increase exponentially for about 3 h, although the cell viability declined rapidly within 30 min of penicillin addition. Prolonged treatment with beta-lactam antibiotics produced cells resistant to these antibiotics. A fluctuation test indicated that this resistance might be due to adaptive mutation. Cells resistant to a beta-lactam exhibited broad cross-resistance to other beta-lactam antibiotics. A new 12,000-Da outer membrane protein was detected both in beta-lactam-resistant cells and in wild-type cells growing in medium containing beta-lactam antibiotics. While the penicillin-resistant cells had all of the penicillin-binding proteins (PBPs) present in the parental cells, significant differences in the relative proportion of low-molecular-weight PBPs were seen. The low-molecular-weight PBPs from resistant cells seemed to form more stable complexes with penicillin than those from the parental strain.<\/jats:p>","DOI":"10.1128\/aac.36.4.788","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:05:50Z","timestamp":1340841950000},"page":"788-795","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Interaction of Vibrio cholerae cells with beta-lactam antibiotics: emergence of resistant cells at a high frequency"],"prefix":"10.1128","volume":"36","author":[{"given":"T K","family":"Sengupta","sequence":"first","affiliation":[{"name":"Biophysics Division, Indian Institute of Chemical Biology, Jadavpur, Calcutta."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Chaudhuri","sequence":"additional","affiliation":[{"name":"Biophysics Division, Indian Institute of Chemical Biology, Jadavpur, Calcutta."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Majumdar","sequence":"additional","affiliation":[{"name":"Biophysics Division, Indian Institute of Chemical Biology, Jadavpur, Calcutta."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Lohia","sequence":"additional","affiliation":[{"name":"Biophysics Division, Indian Institute of Chemical Biology, Jadavpur, Calcutta."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A N","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Biophysics Division, Indian Institute of Chemical Biology, Jadavpur, Calcutta."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Das","sequence":"additional","affiliation":[{"name":"Biophysics Division, Indian Institute of Chemical Biology, Jadavpur, Calcutta."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.36.4.788","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.36.4.788","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T15:24:43Z","timestamp":1645457083000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.36.4.788"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,4]]}},"alternative-id":["10.1128\/AAC.36.4.788"],"URL":"https:\/\/doi.org\/10.1128\/aac.36.4.788","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,4]]},"assertion":[{"value":"1992-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}