{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,8]],"date-time":"2024-09-08T18:13:03Z","timestamp":1725819183786},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1993,2,1]],"date-time":"1993-02-01T00:00:00Z","timestamp":728524800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1993,2]]},"abstract":"<jats:p>Two antiviral compounds, (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (HPMPC) and 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG), were evaluated for their effects on rat cytomegalovirus (RCMV)-induced interstitial pneumonitis after allogeneic bone marrow transplantation (BMTx). Eight-week-old Brown Norway rats immunosuppressed by a lethal dose of total body irradiation were inoculated with RCMV and received allogeneic bone marrow cells from Lewis rats. Animals were treated with either HPMPC (20 mg\/kg of body weight as a single dose) or DHPG (20 mg\/kg as two daily doses for 5 days). The effect of antiviral therapy was monitored by measuring RCMV titers in different organs and the histopathologic changes in lungs at 8 to 10 days postinfection. In RCMV-infected allogeneic BMTx recipients, severe diffuse thickening of alveolar septa (6.02 microns) with a diffuse infiltration of mononuclear cells occurred, whereas in the noninfected allogeneic BMTx recipients, the septal width was on the order of 2 microns (P &lt; 0.01). Treatment with DHPG (20 mg\/kg in two daily doses for 5 days) resulted in a decrease in virus titers (log10 PFU per gram of tissue) in lungs and spleens from 3.81 +\/- 0.34 and 4.29 +\/- 1.07 (untreated animals) to 1.26 +\/- 0.53 and 3.22 +\/- 0.27 (treated animals), respectively. Treatment with HPMPC (20 mg\/kg as a single dose) resulted in a complete reduction of virus titers in all organs to below the detection level (P &lt; 0.01). Furthermore, antiviral treatment resulted in a reduction of the alveolar septal width from 6.02 +\/- 1.59 microns (untreated animals) to 4.67 +\/- 1.70 and 3.32 +\/- 0.63 microns after DHPG and HPMPC treatment, respectively. Treatment with HPMPC (20 mg\/kg as a single dose) resulted in a complete reduction of virus titers in all organs to below the detection level (P &lt;0.01). Furthermore, antiviral treatment resulted in a reduction of the alveolar septal width from 6.02 +\/- 1.59 micrometre (untreated animals) to 4.67 +\/- 0.63 micrometre after DHPG and HPMPC treatment, respectively. Furthermore, the influx of mononuclear cells in the alveolar septa was significantly impaired after treatment with HPMPC (P &lt;0.01). We conclude that in the described rat model, HPMPC is highly effective in suppressing RCMV-induced interstitial pneumonitis after allogeneic BMTx.<\/jats:p>","DOI":"10.1128\/aac.37.2.218","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:05:50Z","timestamp":1340841950000},"page":"218-223","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Rat cytomegalovirus-induced pneumonitis after allogeneic bone marrow transplantation: effective treatment with (S)-1-(3-hydroxy-2-phosphonyl-methoxypropyl)cytosine"],"prefix":"10.1128","volume":"37","author":[{"given":"F S","family":"Stals","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Zeytinoglu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Havenith","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"de Clercq","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C A","family":"Bruggeman","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.37.2.218","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.37.2.218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T15:33:07Z","timestamp":1645457587000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.37.2.218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,2]]}},"alternative-id":["10.1128\/AAC.37.2.218"],"URL":"https:\/\/doi.org\/10.1128\/aac.37.2.218","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,2]]},"assertion":[{"value":"1993-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}