{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T18:38:28Z","timestamp":1766428708294},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1994,2,1]],"date-time":"1994-02-01T00:00:00Z","timestamp":760060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1994,2]]},"abstract":"<jats:p>We compared the experimental toxicities and activities of deoxycholate amphotericin B (d-AmB) dissolved in glucose (Dd-AmB) or mixed with 20% Intralipid (ILd-AmB). In vitro, ILd-AmB against renal tubular cells in primary culture. In vivo, the toxicities and activities of Dd-AmB and ILd-AmB were studied in DBA2 mice with cryptococcosis. The maximum tolerated dose of intravenously administered d-AmB, i.e., the dose that induced less than 15% mortality because of toxicity, was 1.7 to 2.5 times higher when it was administered as ILd-AmB than when it was administered as Dd-AmB. Both treatments given intravenously at the same dose were equivalent for improving the survival of mice and reducing CFU counts in infected tissue, but at maximum tolerated doses, ILd-AmB (2 mg\/kg of body weight) was more effective than Dd-AmB (0.8 to 1.2 mg\/kg). AmB concentrations in spleen, liver, lung, and kidney were measured by high-pressure liquid chromatography 4 and 24 h after a single injection of 1.2 mg of Dd-AmB per kg, 1.2 mg of ILd-AmB per kg, or 2 mg of ILd-AmB per kg. In a given organ, AmB levels were similar after administration of 1.2 mg of Dd-AmB or ILd-AmB per kg but were significantly higher after administration of 2 mg of ILd-AmB per kg. The lower level of toxicity of ILd-AmB might be explained by circular dichroism experiments, showing that ILd-AmB contained 10-fold less soluble oligomeric AmB, which is believed to be the toxic form of the drug, than Dd-AmB. We conclude that ILd-AmB is as efficient as Dd-AmB and is better tolerated than Dd-AmB in mice with experimental cryptococcosis. By allowing higher doses of AmB to be infused, Intralipid enhances AmB concentrations in infected sites, and thus the therapeutic activity of the drug.<\/jats:p>","DOI":"10.1128\/aac.38.2.177","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:05:50Z","timestamp":1340841950000},"page":"177-183","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["In vitro renal toxicity and in vivo therapeutic efficacy in experimental murine cryptococcosis of amphotericin B (Fungizone) associated with Intralipid"],"prefix":"10.1128","volume":"38","author":[{"given":"V","family":"Joly","sequence":"first","affiliation":[{"name":"Laboratoire d'Etude des Infections Exp\u00e9rimentales, Facult\u00e9 X, Bichat, Universit\u00e9 Paris 7, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Farinotti","sequence":"additional","affiliation":[{"name":"Laboratoire d'Etude des Infections Exp\u00e9rimentales, Facult\u00e9 X, Bichat, Universit\u00e9 Paris 7, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Saint-Julien","sequence":"additional","affiliation":[{"name":"Laboratoire d'Etude des Infections Exp\u00e9rimentales, Facult\u00e9 X, Bichat, Universit\u00e9 Paris 7, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Ch\u00e9ron","sequence":"additional","affiliation":[{"name":"Laboratoire d'Etude des Infections Exp\u00e9rimentales, Facult\u00e9 X, Bichat, Universit\u00e9 Paris 7, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Carbon","sequence":"additional","affiliation":[{"name":"Laboratoire d'Etude des Infections Exp\u00e9rimentales, Facult\u00e9 X, Bichat, Universit\u00e9 Paris 7, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Yeni","sequence":"additional","affiliation":[{"name":"Laboratoire d'Etude des Infections Exp\u00e9rimentales, Facult\u00e9 X, Bichat, Universit\u00e9 Paris 7, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.38.2.177","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.38.2.177","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T15:37:51Z","timestamp":1645457871000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.38.2.177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,2]]}},"alternative-id":["10.1128\/AAC.38.2.177"],"URL":"https:\/\/doi.org\/10.1128\/aac.38.2.177","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2]]},"assertion":[{"value":"1994-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}