{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T04:22:28Z","timestamp":1754108548859},"reference-count":0,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[1997,8,1]],"date-time":"1997-08-01T00:00:00Z","timestamp":870393600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1997,8]]},"abstract":"<jats:p>The efficacy of a new liposomal formulation of amphotericin B was compared to that of amphotericin B deoxycholate (Fungizone) in a murine model of visceral leishmaniasis induced by Leishmania infantum. Median effective doses (ED50) were determined with two different strains: strain 1 was obtained from an untreated patient, and strain 2 was obtained from a patient who had received 12.5 g of amphotericin B over 3 years. BALB\/c mice were infected intravenously on day 0 with promastigotes and then treated on days 14, 16, and 18 (strain 1) or on days 21, 23, and 25 (strain 2) with the liposomal formulation of amphotericin B (five doses were tested for each strain: 0.05, 0.1, 0.5, 0.8, and 3 mg\/kg of body weight) or with conventional amphotericin B (four doses were tested for each strain: 0.05, 0.1, 0.5, and 0.8 mg\/kg). Mice in the control group received normal saline solution. The liposomal amphotericin B formulation was about three times more active than the conventional drug against both strains. ED50 of the liposomal formulation were 0.054 (strain 1) and 0.194 (strain 2) mg\/kg. ED50 of conventional amphotericin B were 0.171 (strain 1) and 0.406 (strain 2) mg\/kg. Determination of drug tissular levels, 3 days after the last drug administration, showed a drug accumulation in hepatic and splenic tissues much higher after administration of liposomal amphotericin B than after conventional amphotericin B. A lack of toxicity was noted in all groups treated with the liposomal formulation.<\/jats:p>","DOI":"10.1128\/aac.41.8.1731","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:29:10Z","timestamp":1539044950000},"page":"1731-1734","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Activity of a new liposomal formulation of amphotericin B against two strains of Leishmania infantum in a murine model"],"prefix":"10.1128","volume":"41","author":[{"given":"M","family":"Paul","sequence":"first","affiliation":[{"name":"Laboratoire de Pharmacotechnie, Service Pharmacie, CHU H. Mondor, Creteil, France. alastier@micronet.fr"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Durand","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacotechnie, Service Pharmacie, CHU H. Mondor, Creteil, France. alastier@micronet.fr"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Fessi","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacotechnie, Service Pharmacie, CHU H. Mondor, Creteil, France. alastier@micronet.fr"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Rivollet","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacotechnie, Service Pharmacie, CHU H. Mondor, Creteil, France. alastier@micronet.fr"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Houin","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacotechnie, Service Pharmacie, CHU H. Mondor, Creteil, France. alastier@micronet.fr"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Astier","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacotechnie, Service Pharmacie, CHU H. Mondor, Creteil, France. alastier@micronet.fr"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Deniau","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacotechnie, Service Pharmacie, CHU H. Mondor, Creteil, France. alastier@micronet.fr"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.41.8.1731","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.41.8.1731","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:14:52Z","timestamp":1645474492000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.41.8.1731"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1997,8]]}},"alternative-id":["10.1128\/AAC.41.8.1731"],"URL":"https:\/\/doi.org\/10.1128\/aac.41.8.1731","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8]]},"assertion":[{"value":"1997-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}