{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T06:55:50Z","timestamp":1785308150981,"version":"3.55.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Twelve ceftazidime-resistant isolates of the family\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            (11\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            isolates and 1\n            <jats:italic>Escherichia coli<\/jats:italic>\n            isolate) were collected in 1995 from three Polish hospitals located in different cities. All were identified as producers of extended-spectrum \u03b2-lactamases (ESBLs). Detailed analysis of their \u03b2-lactamase contents revealed that six of them expressed SHV-5-like ESBLs. The remaining six were found to produce three different TEM enzymes, each characterized by a pI value of 6.0 and specified by new combinations of amino acid substitutions. The amino acid substitutions compared to the TEM-1 \u03b2-lactamase sequence were Gly238Ser, Glu240Lys, and Thr265Met for TEM-47; Leu21Phe, Gly238Ser, Glu240Lys, and Thr265Met for TEM-48; and Leu21Phe, Gly238Ser, Glu240Lys, Thr265Met, and Ser268Gly for TEM-49. The new TEM \u03b2-lactamases, TEM-47, TEM-48, and TEM-49, belong to a subfamily of TEM-2-related enzymes. Genes coding for TEM-47 and TEM-49 could have originated from the TEM-48-encoding sequence by various single genetic events. The new TEM derivatives probably document the already advanced microevolution of ESBLs ongoing in Polish hospitals, in a majority of which no monitoring of ESBL producers was performed before 1996.\n          <\/jats:p>","DOI":"10.1128\/aac.42.3.514","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:30:35Z","timestamp":1539045035000},"page":"514-520","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Ceftazidime-Resistant\n            <i>Enterobacteriaceae<\/i>\n            Isolates from Three Polish Hospitals: Identification of Three Novel TEM- and SHV-5-Type Extended-Spectrum \u03b2-Lactamases"],"prefix":"10.1128","volume":"42","author":[{"given":"Marek","family":"Gniadkowski","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Sera &amp; Vaccines Central Research Laboratory, 00-725 Warsaw, Poland,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Schneider","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Max von Pettenkofer-Institut, 80336 Munich, Germany2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renate","family":"Jungwirth","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Max von Pettenkofer-Institut, 80336 Munich, Germany2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleria","family":"Hryniewicz","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Sera &amp; Vaccines Central Research Laboratory, 00-725 Warsaw, Poland,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adolf","family":"Bauernfeind","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Max von Pettenkofer-Institut, 80336 Munich, Germany2"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2510073"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/0014-5793(88)80734-8","article-title":"Single amino acid substitution between SHV-1 \u03b2-lactamase and cefotaxime-hydrolysing SHV-2 enzyme.","volume":"231","author":"Barth\u00e9l\u00e9my M.","year":"1988","unstructured":"Barth\u00e9l\u00e9my M. Peduzzi J. Yaghlane H. B. Labia R. Single amino acid substitution between SHV-1 \u03b2-lactamase and cefotaxime-hydrolysing SHV-2 enzyme. FEBS Lett. 231 1988 217 220","journal-title":"FEBS Lett."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01704610"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01739303"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.3.616"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.12.2439"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1128\/AAC.39.1.145","article-title":"Single amino acid replacements at positions altered in naturally occurring extended-spectrum TEM \u03b2-lactamases.","volume":"39","author":"Blasquez J.","year":"1995","unstructured":"Blasquez J. Morosini M.-I. Negri M.-C. Gonzalez-Leiza M. Baquero F. 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