{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:48:51Z","timestamp":1761101331849},"reference-count":24,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Forty-two men with gonococcal urethritis were treated with an oral dosage of 200 mg of pazufloxacin, a new fluoroquinolone, three times daily for 3 days. Only 28 of the 42 men (66.7%) had negative culture results for\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            during follow-up. Of the 42 isolates, 41 could be recultured for antibiotic susceptibility testing and DNA sequencing. In 26 of the 41 isolates (63.4%), GyrA mutations with or without ParC mutations were identified. Among the 26 isolates, 23 contained a single GyrA mutation, 1 contained two GyrA mutations, and 2 contained three mutations including double GyrA and single ParC mutations. A single Ser-91-to-Phe mutation, which was detected in 14 of the 26 isolates, was the most common GyrA mutation, followed by an Ala-75 to Ser mutation and an Asp-95 to Asn or Gly mutation in GyrA. All three isolates with two or three mutations contained the Ser-91-to-Phe GyrA mutation. Eleven of the 14 isolates with the single Ser-91-to-Phe mutation within GyrA and all 3 isolates with two or three mutations persisted after pazufloxacin treatment. On the other hand, all 15 wild-type and 9 mutant isolates with a substitution at codon Ala-75 or Asp-95 were eradicated. The mean MIC of pazufloxacin for mutants with the single Ser-91-to-Phe mutation in GyrA was 66-fold higher than that for the wild type. The results obtained in this study suggest that a high prevalence of fluoroquinolone-resistant gonococcal isolates with the Ser-91-to-Phe mutation in GyrA reduced the efficacy of pazufloxacin as treatment for gonococcal urethritis.\n          <\/jats:p>","DOI":"10.1128\/aac.42.3.579","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:30:35Z","timestamp":1539045035000},"page":"579-582","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Reduced Clinical Efficacy of Pazufloxacin against Gonorrhea Due to High Prevalence of Quinolone-Resistant Isolates with the GyrA Mutation"],"prefix":"10.1128","volume":"42","author":[{"given":"Masatoshi","family":"Tanaka","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuro","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Misao","family":"Sakumoto","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Saika","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Chemotherapy Division, Mitsubishi-kagaku BCL, Tokyo,2 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Intetsu","family":"Kabayashi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Chemotherapy Division, Mitsubishi-kagaku BCL, Tokyo,2 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joichi","family":"Kumazawa","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"The Pazufloxacin Std","family":"Group","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","unstructured":"Ausubel\n              F. 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