{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:57:22Z","timestamp":1790506642214,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1998,5,1]],"date-time":"1998-05-01T00:00:00Z","timestamp":893980800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1998,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Alveolar echinococcosis, caused by the larval (metacestode) stage of the tapeworm\n            <jats:italic>Echinococcus multilocularis<\/jats:italic>\n            , is a lethal parasitosis of the liver prevalent in the Northern Hemisphere. For chemotherapy the benzimidazole derivatives mebendazole and albendazole were introduced, and their use has resulted in a significant improvement in the survival rates. However, data from experiments with animals and clinical observations indicate that these drugs elicit only parasitostatic activity and in most cases are not able to completely eliminate the parasitic metacestode tissue. In the present study, we applied a culture system for the in vitro growth and proliferation of\n            <jats:italic>E. multilocularis<\/jats:italic>\n            metacestodes to analyze the parasitostatic and parasitocidal potential of mebendazole. Here, we demonstrate for the first time that at concentrations of &gt;0.1 \u03bcM, i.e., at concentrations used for therapy of human alveolar echinococcosis, this antihelminth drug is parasitocidal in vitro. Viability assessment was performed by infection experiments with\n            <jats:italic>Meriones unguiculatus<\/jats:italic>\n            and mebendazole-treated metacestode tissue and by reverse transcription-PCR for the detection of\n            <jats:italic>E. multilocularis<\/jats:italic>\n            mRNA. The\n            <jats:italic>E. multilocularis<\/jats:italic>\n            in vitro model proved to be a valuable tool for the analysis of the potential of antihelminth drugs.\n          <\/jats:p>","DOI":"10.1128\/aac.42.5.1052","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:31:56Z","timestamp":1539045116000},"page":"1052-1056","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["In Vitro Activities of Benzimidazoles against\n            <i>Echinococcus multilocularis<\/i>\n            Metacestodes"],"prefix":"10.1128","volume":"42","author":[{"given":"Heike","family":"Jura","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Hygiene und Mikrobiologie, Universita\u0308t Wu\u0308rzburg, Wu\u0308rzburg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augustinus","family":"Bader","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Leibniz-Institut fu\u0308r Biotechnologie, Medizinische Hochschule Hannover, Hannover,2 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Frosch","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Hygiene und Mikrobiologie, Universita\u0308t Wu\u0308rzburg, Wu\u0308rzburg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1007\/BF01711918","article-title":"Langzeitverlauf bei 60 Patienten mit alveol\u00e4rer Echinokokkose unter Dauertherapie mit Mebendazol.","volume":"66","author":"Ammann R.","year":"1988","unstructured":"Ammann R. Tschudi K. von Ziegler M. Meister F. Cotting J. Eckert J. Witassek F. Langzeitverlauf bei 60 Patienten mit alveol\u00e4rer Echinokokkose unter Dauertherapie mit Mebendazol. Klin. Wochenschr. 66 1988 1060 1073","journal-title":"Klin. Wochenschr."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1002\/hep.1840190328","article-title":"Effect of chemotherapy on the larval mass and on the long-term course of alveolar echinococcosis.","volume":"19","author":"Ammann R.","year":"1993","unstructured":"Ammann R. Ilitsch N. Marincek B. Freiburghaus A. U. Effect of chemotherapy on the larval mass and on the long-term course of alveolar echinococcosis. Hepatology 19 1993 735 742","journal-title":"Hepatology"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1007\/BF00930903","article-title":"Improvement of liver resectional therapy by adjuvant chemotherapy in alveolar hydatid disease.","volume":"77","author":"Ammann R. W.","year":"1991","unstructured":"Ammann R. W. Improvement of liver resectional therapy by adjuvant chemotherapy in alveolar hydatid disease. Parasitol. Res. 77 1991 290 293","journal-title":"Parasitol. Res."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"506","DOI":"10.4269\/ajtmh.1990.43.506","article-title":"Recurrence rate after discontinuation of long-term mebendazole therapy in alveolar echinococcosis (preliminary results).","volume":"43","author":"Ammann R. W.","year":"1990","unstructured":"Ammann R. W. Hirsbrunner R. Cotting J. Steiger U. Jaquier P. Eckert J. Recurrence rate after discontinuation of long-term mebendazole therapy in alveolar echinococcosis (preliminary results). Am J. Trop. Med. Hyg. 43 1990 506 515","journal-title":"Am J. Trop. Med. Hyg."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0169-4758(90)90232-S","article-title":"Use of benzimidazole chemotherapy in human helminthiases: indications and efficacy.","volume":"6","author":"Cook G. C.","year":"1990","unstructured":"Cook G. C. Use of benzimidazole chemotherapy in human helminthiases: indications and efficacy. Parasitol. Today 6 1990 133 136","journal-title":"Parasitol. Today"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1021\/bp00009a007","article-title":"Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration.","volume":"7","author":"Dunn J. C. Y.","year":"1991","unstructured":"Dunn J. C. Y. Tompkins R. G. Yarmush M. L. Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol. Prog. 7 1991 237 245","journal-title":"Biotechnol. Prog."},{"key":"e_1_3_2_8_2","unstructured":"Eckert\n              J.\n              Ammann\n              R. W.\n          Clinical diagnosis and treatment of echinococcosis in humans\n          Echinococcus and hydatid disease.\n              Thompson\n              R. C. A.\n              Lymbery\n              A. J.\n          1995\n          411\n          463\n          CAB International\n          Wallingford England"},{"key":"e_1_3_2_9_2","first-page":"247","article-title":"Zur Wirkung von Mebendazol auf Metazestoden von Mesocestoides corti und Echinococcus multilocularis.","volume":"27","author":"Eckert J.","year":"1976","unstructured":"Eckert J. Pohlenz J. Zur Wirkung von Mebendazol auf Metazestoden von Mesocestoides corti und Echinococcus multilocularis. Tropenmed. Parasitol. 27 1976 247 262","journal-title":"Tropenmed. Parasitol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/0166-6851(91)90108-I","article-title":"Cloning and characterisation of an immunodominant major surface antigen of Echinococcus multilocularis.","volume":"48","author":"Frosch P. M.","year":"1991","unstructured":"Frosch P. M. Frosch M. Pfister T. Schaad V. Bitter-Suermann D. Cloning and characterisation of an immunodominant major surface antigen of Echinococcus multilocularis. Mol. Biol. Parasitol. 48 1991 121 130","journal-title":"Mol. Biol. Parasitol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1017\/S0031182000049738","article-title":"The lethal effect of mebendazole on secondary Echinococcus granulosus cysticerci and Taenis pisiformis and tetrathyridia of Mesocestoides corti.","volume":"70","author":"Heath D. D.","year":"1975","unstructured":"Heath D. D. Christie M. J. Chevis R. A. F. 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The role of the cytoskeletal protein, tubulin, in the mode of action and mechanism of the drug resistance to benzimidazoles. Int. J. Parasitol. 18 1988 885 936","journal-title":"Int. J. Parasitol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(90)90227-U"},{"key":"e_1_3_2_17_2","unstructured":"L\u00f6scher\n              T.\n              von Sonnenburg\n              F.\n              Nothdurft\n              H. D.\n          Epidemiology and therapy of human echinococcosis in central Europe.\n          8th International Congress for Tropical Medicine and Malaria\n          1992"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0168-8278(85)80774-1","article-title":"High oral doses of mebendazole interfere with growth of larval Echinococcus multilocularis lesions.","volume":"1","author":"Luder P. J.","year":"1985","unstructured":"Luder P. J. Robotti G. Meister F. P. Bircher J. High oral doses of mebendazole interfere with growth of larval Echinococcus multilocularis lesions. J. Hepatol. 1 1985 369 377","journal-title":"J. Hepatol."},{"key":"e_1_3_2_19_2","first-page":"443","article-title":"Treatment of hydatid disease with high oral doses of mebendazole. Long-term follow-up of plasma mebendazole levels and drug interactions.","volume":"31","author":"Luder P. J.","year":"1986","unstructured":"Luder P. J. Siffert B. Wittassek F. Meister F. Bircher J. Treatment of hydatid disease with high oral doses of mebendazole. Long-term follow-up of plasma mebendazole levels and drug interactions. Eur. J. Pharmacol. 31 1986 443 448","journal-title":"Eur. J. Pharmacol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00608219"},{"key":"e_1_3_2_21_2","unstructured":"Rausch\n              R. 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F.\n              Maniatis\n              T.\n          Molecular cloning: a laboratory manual.\n          1989\n          Cold Spring Harbor Laboratory Press\n          Cold Spring Harbor N.Y"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/BF00929509","article-title":"Chemotherapy for larval echinococcosis in animals and humans: report of a workshop.","volume":"53","author":"Schantz P. M.","year":"1982","unstructured":"Schantz P. M. Van den Bossche H. Eckert J. Chemotherapy for larval echinococcosis in animals and humans: report of a workshop. Z. Parasitenkunde. 53 1982 5 26","journal-title":"Z. Parasitenkunde."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1093\/infdis\/162.6.1403","article-title":"Effects of albendazole on Echinococcus multilocularis infection in the Mongolian jird.","volume":"162","author":"Schantz P. M.","year":"1990","unstructured":"Schantz P. M. Brandt F. H. Dickinson C. M. Allen C. R. Roberts J. M. Eberhard M. L. Effects of albendazole on Echinococcus multilocularis infection in the Mongolian jird. J. Infect. Dis. 162 1990 1403 1407","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_26_2","unstructured":"Schicker\n              H. J.\n          Die Echinokokkose des Menschen. Stand der Diagnostik Therapie und Prognose bei Echinokokkenerkrankungen in Baden-W\u00fcrttemberg in den Jahren 1960\u201372. Medical dissertation.\n          1976\n          University of T\u00fcbingen\n          T\u00fcbingen Germany"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0091-679X(08)61797-5","article-title":"Preparation of isolated rat liver cells.","volume":"13","author":"Seglen P. O.","year":"1976","unstructured":"Seglen P. O. Preparation of isolated rat liver cells. Methods Cell Biol. 13 1976 29 83","journal-title":"Methods Cell Biol."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.8.1679"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","unstructured":"Van den Bossche\n              H.\n          Mode of action of anticestodal agents\n          Chemotherapy of parasitic diseases.\n              Campbell\n              W. C.\n              Rew\n              R. S.\n          1986\n          139\n          157\n          Plenum Press\n          New York N.Y","DOI":"10.1007\/978-1-4684-1233-8_25"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0169-4758(90)90230-2","article-title":"Resistence in nematode parasites of livestock to the benzimidazole antihelmintics.","volume":"6","author":"Waller P. J.","year":"1990","unstructured":"Waller P. J. Resistence in nematode parasites of livestock to the benzimidazole antihelmintics. Parasitol. Today 6 1990 127 129","journal-title":"Parasitol. Today"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1093\/clinids\/15.2.234","article-title":"Parasitocidal effect of chemotherapy in alveolar hydatid disease: review of experience with mebendazole and albendazole in Alaskan Eskimos.","volume":"15","author":"Wilson J. F.","year":"1992","unstructured":"Wilson J. F. Rausch R. L. McMahon B. J. Schantz P. M. Parasitocidal effect of chemotherapy in alveolar hydatid disease: review of experience with mebendazole and albendazole in Alaskan Eskimos. Clin. Infect. Dis. 15 1992 234 249","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1097\/00000658-199503000-00015","article-title":"Alveolar hydatide disease. Review of the surgical experience in 42 cases of active disease among Alaskan eskimos.","volume":"221","author":"Wilson J. F.","year":"1995","unstructured":"Wilson J. F. Rausch R. L. Wilson F. R. Alveolar hydatide disease. Review of the surgical experience in 42 cases of active disease among Alaskan eskimos. Ann. Surg. 221 1995 315 323","journal-title":"Ann. Surg."}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.42.5.1052","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.42.5.1052","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T22:06:19Z","timestamp":1645481179000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.42.5.1052"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,5]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1998,5]]}},"alternative-id":["10.1128\/AAC.42.5.1052"],"URL":"https:\/\/doi.org\/10.1128\/aac.42.5.1052","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,5]]},"assertion":[{"value":"1997-12-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-02-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}