{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:08:15Z","timestamp":1791148095992,"version":"4.1.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[1998,5,1]],"date-time":"1998-05-01T00:00:00Z","timestamp":893980800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1998,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Enterococcus casseliflavus<\/jats:italic>\n            UC73 is a clinical blood isolate with high-level resistance to gentamicin. DNA preparations from UC73 failed to hybridize with intragenic probes for\n            <jats:italic>aac(6\u2032)-Ie-aph(2\")-Ia<\/jats:italic>\n            and\n            <jats:italic>aph(2\")-Ic<\/jats:italic>\n            . A 4-kb fragment from UC73 was cloned and found to confer resistance to gentamicin in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            DH5\u03b1 transformants. Nucleotide sequence analysis revealed the presence of a 906-bp open reading frame whose deduced amino acid sequence had a region with homology to the aminoglycoside-modifying enzyme APH(2\")-Ic and to the C-terminal domain of the bifunctional enzyme AAC(6\u2032)-APH(2\"). The gene is designated\n            <jats:italic>aph(2\")-Id<\/jats:italic>\n            , and its observed phosphotransferase activity is designated APH(2\")-Id. A PCR-generated intragenic probe hybridized to the genomic DNA from 17 of 118 enterococcal clinical isolates (108 with high-level gentamicin resistance) from five hospitals. All 17 were vancomycin-resistant\n            <jats:italic>Enterococcus faecium<\/jats:italic>\n            isolates, and pulsed-field typing revealed three distinct clones. The combination of ampicillin plus either amikacin or neomycin exhibited synergistic killing against\n            <jats:italic>E. casseliflavus<\/jats:italic>\n            UC73. Screening and interpretation of high-level aminoglycoside resistance in enterococci may need to be modified to include detection of APH(2\")-Id.\n          <\/jats:p>","DOI":"10.1128\/aac.42.5.1229","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:31:56Z","timestamp":1539045116000},"page":"1229-1232","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["A New High-Level Gentamicin Resistance Gene,\n            <i>aph(2\")-Id<\/i>\n            , in\n            <i>Enterococcus<\/i>\n            spp"],"prefix":"10.1128","volume":"42","author":[{"given":"Shane F.","family":"Tsai","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Research and Medical Service, Department of Veterans\u2019 Affairs Medical Center,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus J.","family":"Zervos","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->William Beaumont Hospital, Royal Oak, Michigan 480732;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don B.","family":"Clewell","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Departments of Biologic and Materials Sciences and Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 481093; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan M.","family":"Donabedian","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->William Beaumont Hospital, Royal Oak, Michigan 480732;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel F.","family":"Sahm","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Department of Pathology, Jewish Hospital at Washington University Medical Center, St. Louis, Missouri 631104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph W.","family":"Chow","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Research and Medical Service, Department of Veterans\u2019 Affairs Medical Center,1 and"},{"name":"<!--label omitted: 5-->Wayne State University School of Medicine,5 Detroit, Michigan 48201;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","unstructured":"Arduino\n              R. C.\n              Murray\n              B. E.\n          Enterococci: antimicrobial resistance\n          Update to principles and practice of infectious diseases.\n              Mandell\n              G. L.\n              Douglas\n              R. G.\n              Bennett\n              J. E.\n          1993\n          3\n          15\n          Churchill Livingstone Inc.\n          New York N.Y"},{"key":"e_1_3_2_4_2","unstructured":"Ausubel\n              F. M.\n              Brent\n              R.\n              Kingston\n              R. E.\n              Moore\n              D. D.\n              Seidman\n              J. G.\n              Smith\n              J. A.\n              Struhl\n              K.\n          Current protocols in molecular biology\n          1\n          1995\n          John Wiley & Sons Inc.\n          New York N.Y"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.19.4.549"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.8.1565"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.3.511"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.152.3.1220-1230.1982"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.6.883"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.1Part1.387"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.11.2757-2761.1992"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.1.141-145.1995"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.32.10.1528"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.4.731-734.1989"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.167.2.631-638.1986"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.22.6644-6651.1995"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1172\/JCI109149","article-title":"Aminoglycoside-inactivating enzymes in clinical isolates of Streptococcus faecalis: an explanation for antibiotic synergism.","volume":"62","author":"Krogstad D. J.","year":"1978","unstructured":"Krogstad D. J. Korfhagen R. R. Moellering R. C. Jr. Wennersten C. Swartz M. N. Aminoglycoside-inactivating enzymes in clinical isolates of Streptococcus faecalis: an explanation for antibiotic synergism. J. Clin. Invest. 62 1978 480 486","journal-title":"J. Clin. Invest."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1093\/clind\/15.3.495","article-title":"Resistance of enterococci to aminoglycosides and glycopeptides.","volume":"15","author":"Leclercq R.","year":"1992","unstructured":"Leclercq R. Dutka-Malen S. Brisson-No\u00ebl A. Molinas C. Derlot E. Arthur M. Duval J. Courvalin P. Resistance of enterococci to aminoglycosides and glycopeptides. Clin. Infect. Dis. 15 1992 495 501","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.16.5.598"},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0732-8893(93)90026-4","article-title":"In vitro activity of sparfloxacin and clinafloxacin against multidrug-resistant enterococci.","volume":"17","author":"Perri M. B.","year":"1993","unstructured":"Perri M. B. Chow J. W. Zervos M. J. In vitro activity of sparfloxacin and clinafloxacin against multidrug-resistant enterococci. Diagn. Microbiol. Infect. Dis. 17 1993 151 155","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.5.1194"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1006\/plas.1993.1055","article-title":"Characterization of left four kilobases of conjugative transposon Tn916: determinants involved in excision.","volume":"30","author":"Su Y. A.","year":"1993","unstructured":"Su Y. A. Clewell D. B. Characterization of left four kilobases of conjugative transposon Tn916: determinants involved in excision. Plasmid 30 1993 234 250","journal-title":"Plasmid"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.9.2233-2239.1995"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.5.1152"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(83)90022-7"},{"key":"e_1_3_2_26_2","unstructured":"Tsai\n              S.\n              Zervos\n              M. J.\n              Clewell\n              D. B.\n              Shaw\n              K. J.\n              Sahm\n              D. F.\n              Chow\n              J. 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A.\n          Antibacterial susceptibility tests: dilution and disk diffusion methods\n          Manual of clinical microbiology\n          6th ed.\n              Murray\n              P. R.\n              Baron\n              E. J.\n              Pfaller\n              M. A.\n              Tenover\n              F. C.\n              Yolken\n              R. H.\n          1995\n          1327\n          1341\n          American Society for Microbiology\n          Washington D.C"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.42.5.1229","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.42.5.1229","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:59:54Z","timestamp":1645480794000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.42.5.1229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,5]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1998,5]]}},"alternative-id":["10.1128\/AAC.42.5.1229"],"URL":"https:\/\/doi.org\/10.1128\/aac.42.5.1229","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,5]]},"assertion":[{"value":"1997-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-02-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}