{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T12:58:00Z","timestamp":1773147480923,"version":"3.50.1"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1998,6,1]],"date-time":"1998-06-01T00:00:00Z","timestamp":896659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Antimicrobial susceptibility testing by the ATP-bioluminescence method has been noted for its speed; it provides susceptibility results within 2 to 5 h. However, several disagreements between the ATP method and standard methodology have been reported. The present paper describes a novel ATP method in a 3.5-h test which overcomes these deficiencies through the elimination of false-resistance discrepancies in tests on gram-negative bacteria with \u03b2-lactam agents. In our test model using\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            and piperacillin, it was shown that ATP in filamentous cells accounted for the false resistance. We found that 0.5% 2-amino-2-methyl-1,3-propanediol (AMPD) extracted ATP from the filamentous cells without affecting normal cells and that 0.3 U of adenosine phosphate deaminase (APDase)\/ml simultaneously digested the extracted ATP. We used the mixture of these reagents for the pretreatment of cells in a procedure we named filamentous cell treatment, prior to ATP measurements. This novel ATP method with the filamentous cell treatment eliminated false-resistance discrepancies in tests on\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            with \u03b2-lactam agents, including piperacillin, cefoperazone, aztreonam, imipenem-cilastatin, ceftazidime, and cefsulodin. Furthermore, this novel methodology produced results which agreed with those of the standard microdilution method in other tests on gram-negative and gram-positive bacteria, including\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            ,\n            <jats:italic>Escherichia coli<\/jats:italic>\n            ,\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            , and\n            <jats:italic>Enterococcus faecalis<\/jats:italic>\n            , for non-\u03b2-lactam agents, such as fosfomycin, ofloxacin, minocycline, and aminoglycosides. MICs obtained by the novel ATP method were also in agreement with those obtained by the agar dilution method of susceptibility testing. From these results, it was shown that the novel ATP method could be used successfully to test the activities of antimicrobial agents with the elimination of the previously reported discrepancies.\n          <\/jats:p>","DOI":"10.1128\/aac.42.6.1406","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:32:51Z","timestamp":1539045171000},"page":"1406-1411","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Novel Antibiotic Susceptibility Tests by the ATP-Bioluminescence Method Using Filamentous Cell Treatment"],"prefix":"10.1128","volume":"42","author":[{"given":"Noriaki","family":"Hattori","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Research and Development Division, Kikkoman Corporation, Chiba 278-0005,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moto-O","family":"Nakajima","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Research and Development Division, Kikkoman Corporation, Chiba 278-0005,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"O\u2019Hara","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Division of Microbial Chemistry, Faculty of Pharmaceutical Sciences, Chiba University, Chiba 263-8522,2Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Sawai","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Division of Microbial Chemistry, Faculty of Pharmaceutical Sciences, Chiba University, Chiba 263-8522,2Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1093\/jac\/9.5.343","article-title":"Rapid determination of bacterial susceptibility to antimicrobial agents by a semi-automated continuous flow method.","volume":"9","author":"Bascomb S.","year":"1982","unstructured":"Bascomb S. Glynn A. A. Gaya H. Spencer R. C. Shine P. J. Rapid determination of bacterial susceptibility to antimicrobial agents by a semi-automated continuous flow method. J. Antimicrob. Chemother. 9 1982 343 355","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02013394"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/0958-6946(94)90038-8","article-title":"ATP-bioluminescence techniques for assessing the hygienic condition of milk transport tankers.","volume":"4","author":"Bell C.","year":"1994","unstructured":"Bell C. Stallard P. A. Brown S. E. Standley J. T. E. ATP-bioluminescence techniques for assessing the hygienic condition of milk transport tankers. Int. Dairy J. 4 1994 629 640","journal-title":"Int. Dairy J."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.12.1.61"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.2.5.360"},{"key":"e_1_3_2_7_2","unstructured":"Gutekunst\n              R. R.\n          Rapid procedure under development and evaluation: bioluminescence and impedance measurement\n          The clinical laboratory as an aid in chemotherapy of infectious disease.\n              Bond\n              A.\n              Bartola\n              J. T.\n              Prier\n              J. E.\n          1976\n          85\n          100\n          Williams and Wilkins\n          Baltimore Md"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.166.3901.122"},{"key":"e_1_3_2_9_2","unstructured":"Hojer H. L. Nilsson S. Ansehn and A. Thore. 1976. In-vitro effect of doxycycline on levels of adenosine triphosphate in bacterial cultures. Scand. J. Infect. 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Enhancement of thermostability of firefly luciferase from Luciola lateralis by a single amino acid substitution. Biosci. Biotechnol. Biochem. 58 1994 1170 1171 (Note.)","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.5.1038"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1099\/00222615-35-2-89","article-title":"Antimicrobial susceptibility testing of mycoplasmas by ATP bioluminescence.","volume":"35","author":"Limb D. I.","year":"1991","unstructured":"Limb D. I. Wheat P. F. Hastings J. G. M. Spencer R. C. Antimicrobial susceptibility testing of mycoplasmas by ATP bioluminescence. J. Med. Microbiol. 35 1991 89 92","journal-title":"J. Med. Microbiol."},{"key":"e_1_3_2_17_2","unstructured":"Lundin\n              A.\n              Anson\n              J.\n              Kau\n              P.\n          ATP extractants neutralised by cyclodextrins\n          Proceedings of the 8th International Symposium on Bioluminescence and Chemiluminescence\n          1994\n          399\n          402"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.11.2583"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/j.1365-2672.1984.tb04706.x","article-title":"Determination of susceptibility of Staphylococcus aureus to methicillin by luciferin-luciferase assay of bacterial adenosine triphosphate.","volume":"56","author":"McWalter P. W.","year":"1984","unstructured":"McWalter P. W. Determination of susceptibility of Staphylococcus aureus to methicillin by luciferin-luciferase assay of bacterial adenosine triphosphate. J. Appl. Bacteriol. 56 1984 145 150","journal-title":"J. Appl. Bacteriol."},{"key":"e_1_3_2_20_2","unstructured":"McWalter\n              P. W.\n          Rapid susceptibility testing of Staphylococcus aureus Analytical application of bioluminescence and chemiluminescence.\n              Kricka\n              L. J.\n              Stanley\n              P. E.\n              Thorpe\n              G. H. G.\n              Whitehead\n              T. P.\n          1984\n          17\n          20\n          Academic Press Inc.\n          London United Kingdom"},{"key":"e_1_3_2_21_2","unstructured":"National Committee for Clinical Laboratory Standards\n          Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically 2nd ed. Approved standard M7-A2.\n          1990\n          National Committee for Clinical Laboratory Standards\n          Villanova Pa"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.32.8.1208"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.6.1223-1224.1988"},{"key":"e_1_3_2_24_2","unstructured":"Porter\n              I. A.\n              Reid\n              T. M. S.\n              Wood\n              W. J.\n              Gibson\n              D. M.\n              Hobbs\n              G.\n          Conductance measurements for determining antibiotic sensitivity\n          Antibiotics: assessment of antimicrobial activity and resistance.\n              Russell\n              A. D.\n              Quesnel\n              L. B.\n          1983\n          49\n          60\n          Academic Press\n          London United Kingdom"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0168-1605(93)90098-2","article-title":"Assessment of cleaning and disinfection in the food industry with the rapid ATP-bioluminescence technique combined with the tissue fluid contamination test and a conventional microbiological method.","volume":"20","author":"Poulis J. A.","year":"1993","unstructured":"Poulis J. A. de Pijper M. Mossel D. A. A. Dekkers P. P. A. Assessment of cleaning and disinfection in the food industry with the rapid ATP-bioluminescence technique combined with the tissue fluid contamination test and a conventional microbiological method. Int. J. Food Microbiol. 20 1993 109 116","journal-title":"Int. J. 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