{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:11:25Z","timestamp":1791277885939,"version":"4.1.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1998,7,1]],"date-time":"1998-07-01T00:00:00Z","timestamp":899251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1998,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The contributions of 23 insertion, deletion, or missense mutations within an 81-bp fragment of\n            <jats:italic>rpoB<\/jats:italic>\n            , the gene encoding the \u03b2-subunit of the DNA-dependent RNA polymerase of\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            , to the development of resistance to rifamycins (rifampin, rifabutin, rifapentine, and KRM-1648) in 29 rifampin-resistant clinical isolates were defined. Specific mutant\n            <jats:italic>rpoB<\/jats:italic>\n            alleles led to the development of cross-resistance to all rifamycins tested, while a subset of mutations were associated with resistance to rifampin and rifapentine but not to KRM-1648 or rifabutin. To further study the impact of specific\n            <jats:italic>rpoB<\/jats:italic>\n            mutant alleles on the development of rifamycin resistance, mutations were incorporated into the\n            <jats:italic>rpoB<\/jats:italic>\n            gene of\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            H37Rv, contained on a mycobacterial shuttle plasmid, by in vitro mutagenesis. Recombinant\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            clones containing plasmids with specific mutations in either codon 531 or 526 of\n            <jats:italic>rpoB<\/jats:italic>\n            exhibited high-level resistance to all rifamycins tested, whereas clones containing a plasmid with a mutation in codon 516 exhibited high-level resistance to rifampin and rifapentine but were susceptible to both rifabutin and KRM-1648. These results provided additional proof of the association of specific\n            <jats:italic>rpoB<\/jats:italic>\n            mutations with the development of rifamycin resistance and corroborate previous reports of the usefulness of\n            <jats:italic>rpoB<\/jats:italic>\n            genotyping for predicting rifamycin-resistant phenotypes.\n          <\/jats:p>","DOI":"10.1128\/aac.42.7.1853","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:33:03Z","timestamp":1539045183000},"page":"1853-1857","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":193,"title":["Contribution of\n            <i>rpoB<\/i>\n            Mutations to Development of Rifamycin Cross-Resistance in\n            <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1128","volume":"42","author":[{"given":"D. L.","family":"Williams","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Gillis W. Long Hansen\u2019s Disease Research Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Spring","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Gillis W. Long Hansen\u2019s Disease Research Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Collins","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Gillis W. Long Hansen\u2019s Disease Research Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. P.","family":"Miller","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, New Jersey2;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. B.","family":"Heifets","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->National Jewish Medical and Research Center, Denver, Colorado3; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. R. J.","family":"Gangadharam","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Mycobacteriology Research Laboratory, Section of Infectious Diseases, Department of Medicine, University of Illinois at Chicago, Chicago, Illinois4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. P.","family":"Gillis","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Gillis W. Long Hansen\u2019s Disease Research Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana1;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1093\/jac\/35.2.345","article-title":"Mutation position and type of substitution in the \u03b2-subunit of the RNA polymerase influence in-vitro activity of rifamycins in rifampicin-resistant Mycobacterium tuberculosis.","volume":"35","author":"Bodmer T.","year":"1995","unstructured":"Bodmer T. Zurcher G. Imboden P. Telenti A. Mutation position and type of substitution in the \u03b2-subunit of the RNA polymerase influence in-vitro activity of rifamycins in rifampicin-resistant Mycobacterium tuberculosis. J. Antimicrob. Chemother. 35 1995 345 348","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8479(95)90009-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/141.3.626"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0962-8479(92)90081-T","article-title":"A controlled study of rifabutin and an uncontrolled study of ofloxacin in the treatment of patients with pulmonary tuberculosis resistant to isoniazid, streptomycin and rifampicin.","volume":"73","author":"Hong Kong Chest Service and British Medical Research Council","year":"1992","unstructured":"Hong Kong Chest Service and British Medical Research Council A controlled study of rifabutin and an uncontrolled study of ofloxacin in the treatment of patients with pulmonary tuberculosis resistant to isoniazid, streptomycin and rifampicin. Tubercle Lung Dis. 73 1992 59 67","journal-title":"Tubercle Lung Dis."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.2.440"},{"key":"e_1_3_2_7_2","unstructured":"Maniatis\n              T.\n              Fritsch\n              E. F.\n              Sambrook\n              J.\n          Large-scale isolation of plasmid DNA: lysis by alkali\n          Molecular Cloning: a laboratory manual.\n          1982\n          90\n          91\n          Cold Spring Harbor Laboratory Press\n          Cold Spring Harbor N.Y"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.4.805"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.11.2655"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.496"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/24.5.894"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.4.1053"},{"key":"e_1_3_2_13_2","first-page":"859","article-title":"Routine rapid Mycobacterium species assignment based on species-specific allelic variation in the 65-kilodalton heat shock protein gene (hsp65).","volume":"121","author":"Pai S.","year":"1997","unstructured":"Pai S. Esen N. Pan X. Musser J. M. Routine rapid Mycobacterium species assignment based on species-specific allelic variation in the 65-kilodalton heat shock protein gene (hsp65). Arch. Pathol. Lab. Med. 121 1997 859 864","journal-title":"Arch. Pathol. Lab. Med."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/S0962-8479(96)90030-1","article-title":"Chemotherapeutic activity of benzoxazinorifamycin KRM-1648 against Mycobacterium tuberculosis in C57BL\/6 mice.","volume":"77","author":"Reddy M. V.","year":"1996","unstructured":"Reddy M. V. Luna-Herrera J. Daneluzzi D. Gangadharum P. R. Chemotherapeutic activity of benzoxazinorifamycin KRM-1648 against Mycobacterium tuberculosis in C57BL\/6 mice. Tubercle Lung Dis. 77 1996 154 159","journal-title":"Tubercle Lung Dis."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1093\/jac\/32.6.867","article-title":"The early bactericidal activity of rifabutin in patients with pulmonary tuberculosis measured by sputum viable counts: a new method of drug assessment.","volume":"2","author":"Sirgel D. A.","year":"1993","unstructured":"Sirgel D. A. Botha F. J. Parkin D. P. The early bactericidal activity of rifabutin in patients with pulmonary tuberculosis measured by sputum viable counts: a new method of drug assessment. J. Antimicrob. Chemother. 2 1993 867 875","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/351456a0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90417-F"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.10.2380"},{"key":"e_1_3_2_19_2","unstructured":"Williams\n              D. L.\n              Spring\n              L.\n              Collins\n              L.\n              Miller\n              L.\n              Heifets\n              L.\n              Gillis\n              T. P.\n          Mutations in rpoB and rifamycin cross-resistance in Mycobacterium tuberculosis abstr. 14\n          Program and abstracts of the 32nd Joint Leprosy\/Tuberculosis Conference. U.S.-Japan Cooperative Medical Science Program Cleveland Ohio\n          1997\n          226"},{"key":"e_1_3_2_20_2","unstructured":"Williams\n              D. L.\n              Limbers\n              C. W.\n              Spring\n              L.\n              Jayachandra\n              S.\n              Gillis\n              T. P.\n          PCR-heteroduplex detection of rifampin-resistant Mycobacterium tuberculosis PCR protocols for emerging infectious diseases: a supplement to diagnostic molecular microbiology: principles and applications.\n              Persing\n              D. H.\n          1996\n          122\n          129\n          ASM Press\n          Washington D.C"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1086\/516313","article-title":"Evaluation of polymerase chain reaction-based universal heteroduplex generator assay for direct detection of rifampin susceptibility of Mycobacterium tuberculosis from sputum specimens.","volume":"26","author":"Williams D. L.","year":"1998","unstructured":"Williams D. L. Spring L. Salfinger M. Gillis T. P. Persing D. H. Evaluation of polymerase chain reaction-based universal heteroduplex generator assay for direct detection of rifampin susceptibility of Mycobacterium tuberculosis from sputum specimens. Clin. Infect. Dis. 26 1998 446 450","journal-title":"Clin. Infect. Dis."}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.42.7.1853","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.42.7.1853","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T16:14:05Z","timestamp":1645460045000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.42.7.1853"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,7]]},"references-count":20,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1998,7]]}},"alternative-id":["10.1128\/AAC.42.7.1853"],"URL":"https:\/\/doi.org\/10.1128\/aac.42.7.1853","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,7]]},"assertion":[{"value":"1997-12-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-05-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}