{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:34:50Z","timestamp":1791264890350,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[1999,11,1]],"date-time":"1999-11-01T00:00:00Z","timestamp":941414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In a survey of resistance to amoxicillin among clinical isolates of\n            <jats:italic>Proteus mirabilis<\/jats:italic>\n            , 10 TEM-type \u03b2-lactamases were characterized: (i) the well-known penicillinases TEM-1 and TEM-2, the extended-spectrum \u03b2-lactamases (ESBLs) TEM-3 and TEM-24, and the inhibitor-resistant TEM (IRT) TEM-44 and (ii) five novel enzymes, a penicillinase TEM-57 similar to TEM-1, an ESBL TEM-66 similar to TEM-3, and three IRTs, TEM-65, TEM-73, and TEM-74. The penicillinase TEM-57 and the ESBL TEM-66 differed from TEM-1 and TEM-3, respectively, by the amino acid substitution Gly-92\u2192Asp (nucleotide mutation G-477\u2192A). This substitution could have accounted for the decrease in pIs (5.2 for TEM-57 and 6.0 for TEM-66) but did not necessarily affect the intrinsic activities of these enzymes. The IRT TEM-65 was an IRT-1-like IRT (Cys-244) related to TEM-2 (Lys-39). The two other IRTs, TEM-73 and TEM-74, were related to IRT-1 (Cys-244) and IRT-2 (Ser-244), respectively, and harbored the amino acid substitutions Leu-21\u2192Phe and Thr-265\u2192Met. In this study, the ESBLs TEM-66, TEM-24, and TEM-3 were encoded by large (170- to 180-kb) conjugative plasmids that exhibited similar patterns after digestion and hybridization with the TEM and AAC(6\u2032)I probes. The three IRTs TEM-65, TEM-73, and TEM-74 were encoded by plasmids that ranged in size from 42 to 70 kb but for which no transfer was obtained. The characterization of five new plasmid-mediated TEM-type \u03b2-lactamases and the first report of TEM-24 in\n            <jats:italic>P. mirabilis<\/jats:italic>\n            are evidence of the wide diversity of \u03b2-lactamases produced in this species and of its possible role as a \u03b2-lactamase-encoding plasmid reservoir.\n          <\/jats:p>","DOI":"10.1128\/aac.43.11.2671","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:33:39Z","timestamp":1539045219000},"page":"2671-2677","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Diversity of TEM Mutants in\n            <i>Proteus mirabilis<\/i>"],"prefix":"10.1128","volume":"43","author":[{"given":"R.","family":"Bonnet","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie, Faculte\u0301 de Me\u0301decine, 63001 Clermont-Ferrand Cedex,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"De Champs","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie, Faculte\u0301 de Me\u0301decine, 63001 Clermont-Ferrand Cedex,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Sirot","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie, Faculte\u0301 de Me\u0301decine, 63001 Clermont-Ferrand Cedex,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Chanal","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie, Faculte\u0301 de Me\u0301decine, 63001 Clermont-Ferrand Cedex,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Labia","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->UMR 175, CNRS-MNHN, 29000 Quimper,2 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Sirot","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie, Faculte\u0301 de Me\u0301decine, 63001 Clermont-Ferrand Cedex,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.3.616"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.2.509"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.125.1.149-157.1976"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/38.2.183"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.5.1110"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00006121"},{"key":"e_1_3_2_10_2","unstructured":"Chanal\n              C.\n              Bonnet\n              R.\n              De Champs\n              C.\n              Romaszko\n              J. P.\n              Sirot\n              D.\n              Sirot\n              J.\n          Surveillance de la resistance aux \u03b2-lactamases et diversite moleculaire des enzymes de type penicillinase chez Proteus mirabilis. In Program and abstracts of the 18th Interdisciplinary Meeting on Anti-Infections Chemotherapy.\n          1998\n          Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie\n          Paris France"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/38.1.127"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.33.11.1915"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.2.913-916.1987"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/27.4.441"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/32.4.595"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90540-R"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/34.5.707"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.1.164"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340160406"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.145.3.1365-1373.1981"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(73)80154-1"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/30.4.429"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0147-619X(90)90041-A","article-title":"Direct sequencing of the amplified structural gene and promoter for the extended broad-spectrum \u03b2-lactamase TEM-9 (RHH-1) of Klebsiella pneumoniae.","volume":"23","author":"Mabilat C.","year":"1990","unstructured":"Mabilat C. Goussard S. Sougakoff W. Spencer R. C. Courvalin P. Direct sequencing of the amplified structural gene and promoter for the extended broad-spectrum \u03b2-lactamase TEM-9 (RHH-1) of Klebsiella pneumoniae. Plasmid 23 1990 27 34","journal-title":"Plasmid"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/BF00286188","article-title":"A new example of physical linkage between Tn1 and Tn21: the antibiotic multiple resistance region of plasmid pCFF04 encoding extended-spectrum beta-lactamase TEM-3.","volume":"235","author":"Mabilat C.","year":"1992","unstructured":"Mabilat C. Lourencao-Vital J. Goussard S. Courvalin P. A new example of physical linkage between Tn1 and Tn21: the antibiotic multiple resistance region of plasmid pCFF04 encoding extended-spectrum beta-lactamase TEM-3. Mol. Gen. Genet. 235 1992 113 121","journal-title":"Mol. Gen. 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Infect. 3 (Suppl. 2) 1997 74 75","journal-title":"Clin. Microbiol. Infect."},{"key":"e_1_3_2_27_2","unstructured":"Pagani\n              L.\n              Luzzaro\n              F.\n              Migliavacca\n              R.\n              Perilli\n              M. G.\n              Daturi\n              R.\n              Lombardi\n              G.\n              Matti\n              C.\n              Giacobone\n              E.\n              Amicosante\n              G.\n          Detection and characterization of extended spectrum \u03b2-lactamases in clinical isolates of Proteus mirabilis from North Italian Hospitals abstr. 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