{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:13:47Z","timestamp":1790388827729,"version":"4.1.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[1999,11,1]],"date-time":"1999-11-01T00:00:00Z","timestamp":941414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Mutations of the\n            <jats:italic>rpoB<\/jats:italic>\n            gene conferring resistance to rifampin were analyzed in 40 methicillin-resistant\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            isolates obtained from six countries. Interestingly, the majority of clinical isolates showed multiple mutations within\n            <jats:italic>rpoB<\/jats:italic>\n            . The amino acid substitution 481His\u2192Asn was the most prevalent one, capable of conferring low-level resistance on its own. Cross-resistance to rifampin, rifabutin, and rifapentine was demonstrated for all mutants identified. The level of resistance to rifamycins correlated with both the mutation position and type of amino acid substitution.\n          <\/jats:p>","DOI":"10.1128\/aac.43.11.2813","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:33:39Z","timestamp":1539045219000},"page":"2813-2816","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":100,"title":["Molecular Characterization of\n            <i>rpoB<\/i>\n            Mutations Conferring Cross-Resistance to Rifamycins on Methicillin-Resistant\n            <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"43","author":[{"given":"Thomas A.","family":"Wichelhaus","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institute of Medical Microbiology, University Hospital of Frankfurt, Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Scha\u0308fer","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute of Medical Microbiology, University Hospital of Frankfurt, Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Brade","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute of Medical Microbiology, University Hospital of Frankfurt, Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Bo\u0308ddinghaus","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute of Medical Microbiology, University Hospital of Frankfurt, Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4781(95)00054-K"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.10.2590"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1093\/jac\/35.2.345","article-title":"Mutation position and type of substitution in the \u03b2-subunit of the RNA polymerase influence in-vitro activity of rifamycins in rifampicin-resistant Mycobacterium tuberculosis.","volume":"35","author":"Bodmer T.","year":"1995","unstructured":"Bodmer T. Z\u00fcrcher G. Imboden P. Telenti A. Mutation position and type of substitution in the \u03b2-subunit of the RNA polymerase influence in-vitro activity of rifamycins in rifampicin-resistant Mycobacterium tuberculosis. J. Antimicrob. Chemother. 35 1995 345 348","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.6.1256"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.781"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.5.1118"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.3.414"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(88)90517-7"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.Supplement_1.S3"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)11192-2"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.4.805"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.137.1.374-383.1979"},{"key":"e_1_3_2_14_2","unstructured":"National Committee for Clinical Laboratory Standards\n          Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically\n          4th ed.\n          1997 Approved standard M7-A4. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1111\/j.1432-1033.1981.tb05381.x","article-title":"The primary structure of Escherichia coli RNA polymerase.","volume":"116","author":"Ovchinnikov Y. A.","year":"1981","unstructured":"Ovchinnikov Y. A. Monastyrskaya G. S. Gubanov V. V. Guryev S. O. Chertov O. Y. Modyanov N. N. Grinkevich V. A. Makarova I. A. Marchenko T. V. Polovnikova I. N. Lipkin V. M. Sverdlov E. D. The primary structure of Escherichia coli RNA polymerase. Eur. J. Biochem. 116 1981 621 629","journal-title":"Eur. J. Biochem."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/jac\/41.3.325","article-title":"Alternatives to vancomycin in treating methicillin-resistant Staphylococcus aureus infections.","volume":"41","author":"Rahman M.","year":"1998","unstructured":"Rahman M. Alternatives to vancomycin in treating methicillin-resistant Staphylococcus aureus infections. J. Antimicrob. Chemother. 41 1998 325 328","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(97)00027-7"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"29428","DOI":"10.1074\/jbc.270.49.29428","article-title":"The \u03b2-subunit rif-cluster I is only angstroms away from the active center of Escherichia coli RNA polymerase.","volume":"270","author":"Severinov K.","year":"1995","unstructured":"Severinov K. Mustaev A. Severinova E. Kozlov M. Darst S. A. Goldfarb A. The \u03b2-subunit rif-cluster I is only angstroms away from the active center of Escherichia coli RNA polymerase. J. Biol. Chem. 270 1995 29428 29432","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82407-3"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00283512"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08515.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90417-F"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02026127"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/5.Supplement_3.S407"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1007\/BF01700555","article-title":"Clonal heterogeneity, distribution, and pathogenicity of methicillin-resistant Staphylococcus aureus.","volume":"16","author":"Wichelhaus T. A.","year":"1997","unstructured":"Wichelhaus T. A. Schulze J. Hunfeld K. P. Sch\u00e4fer V. Brade V. Clonal heterogeneity, distribution, and pathogenicity of methicillin-resistant Staphylococcus aureus. Eur. J. Clin. Microbiol. Infect. Dis. 16 1997 893 897","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.7.1853"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/42.5.621"},{"key":"e_1_3_2_28_2","unstructured":"Yao\n              J. D. C.\n              Moellering\n              R. C.\n          Antibacterial agents\n          Manual of clinical microbiology\n          6th ed.\n              Murray\n              P. R.\n              Baron\n              E. J.\n              Pfaller\n              M. A.\n              Tenover\n              F. C.\n              Yolken\n              R. H.\n          1995\n          1281\n          1307\n          American Society for Microbiology\n          Washington D.C."}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.43.11.2813","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.43.11.2813","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T16:01:54Z","timestamp":1645459314000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.43.11.2813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,11]]},"references-count":27,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1999,11]]}},"alternative-id":["10.1128\/AAC.43.11.2813"],"URL":"https:\/\/doi.org\/10.1128\/aac.43.11.2813","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,11]]},"assertion":[{"value":"1999-02-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-08-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}