{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T04:08:49Z","timestamp":1768795729248,"version":"3.49.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1999,2,1]],"date-time":"1999-02-01T00:00:00Z","timestamp":917827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1999,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We investigated the relationship between susceptibility to \u03b2-lactam antibiotics and variation in the major outer membrane protein P2 (OmpP2; also called porin) of persistent nonencapsulated\n            <jats:italic>Haemophilus influenzae<\/jats:italic>\n            isolated from cystic fibrosis patients. Nine OmpP2 variants were selected from two distinct\n            <jats:italic>H. influenzae<\/jats:italic>\n            strains from two patients extensively treated with \u03b2-lactam antibiotics. The variants differed in their susceptibilities to at least two \u03b2-lactam antibiotics. By detergent extraction and column chromatography, OmpP2 was purified from two variants that were derived from strain 70 and that differed notably in their susceptibilities to \u03b2-lactam antibiotics. The proteins were reconstituted into black lipid membranes for measurement of porin function. OmpP2 from the more resistant isolate (isolate 70b) had a smaller channel conductance than OmpP2 of the more susceptible isolate (isolate 70f). DNA sequencing of\n            <jats:italic>ompP2<\/jats:italic>\n            of these isolates revealed single nonsynonymous base differences; there were changes in the amino acid sequence corresponding to surface-exposed loops 4, 5, 6, and 8. Changes in loops 4, 5, and 6 were previously shown to result in antigenic differences. Beside these mutations, variants of strain 70 showed additional mutations in loop 1 and nonexposed loop 3. Taken together, our results suggest that in variants of strain 70, nonsynonymous point mutations accumulated both in the sequences of\n            <jats:italic>ompP2<\/jats:italic>\n            coding for antigen-variable loops and in other loops, notably, loops 1 and 3. The latter changes are suggested to affect the permeability of the porin channel.\n          <\/jats:p>","DOI":"10.1128\/aac.43.2.226","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:34:13Z","timestamp":1539045253000},"page":"226-232","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Variation in the Composition and Pore Function of Major Outer Membrane Pore Protein P2 of\n            <i>Haemophilus influenzae<\/i>\n            from Cystic Fibrosis Patients"],"prefix":"10.1128","volume":"43","author":[{"given":"Annette G.","family":"Regelink","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Dahan","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology and Immunology, McGill University, Montre\u0301al, Que\u0301bec, Canada H3A 2B42"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lieke V. M.","family":"Mo\u0308ller","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam,1 and"},{"name":"<!--label omitted: 3-->Department of Medical Microbiology, University Hospital Groningen, and Laboratory of Public Health Groningen, Groningen,3 The Netherlands, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James W.","family":"Coulton","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology and Immunology, McGill University, Montre\u0301al, Que\u0301bec, Canada H3A 2B42"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Eijk","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Van Ulsen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob","family":"Dankert","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loek","family":"Van Alphen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, Amsterdam,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.8.2033-2037.1992"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.6.2639-2643.1994"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/0022-2836(88)90121-0","article-title":"Mutations that alter the pore function of the OmpF porin of Escherichia coli K12.","volume":"203","author":"Benson S. 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