{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T05:05:47Z","timestamp":1791003947366,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1999,4,1]],"date-time":"1999-04-01T00:00:00Z","timestamp":922924800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1999,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Shotgun cloning experiments with restriction enzyme-digested genomic DNA from\n            <jats:italic>Morganella morganii<\/jats:italic>\n            1, which expresses high levels of cephalosporinase, into the pBKCMV cloning vector gave a recombinant plasmid, pPON-1, which encoded four entire genes:\n            <jats:italic>ampC<\/jats:italic>\n            ,\n            <jats:italic>ampR<\/jats:italic>\n            , an\n            <jats:italic>hybF<\/jats:italic>\n            family gene, and\n            <jats:italic>orf-1<\/jats:italic>\n            of unknown function. The deduced AmpC \u03b2-lactamase of pI 7.6 shared structural and functional homologies with AmpC from\n            <jats:italic>Citrobacter freundii<\/jats:italic>\n            ,\n            <jats:italic>Escherichia coli<\/jats:italic>\n            ,\n            <jats:italic>Yersinia enterocolitica<\/jats:italic>\n            ,\n            <jats:italic>Enterobacter cloacae<\/jats:italic>\n            , and\n            <jats:italic>Serratia marcescens<\/jats:italic>\n            . The overlapping promoter organization of\n            <jats:italic>ampC<\/jats:italic>\n            and\n            <jats:italic>ampR<\/jats:italic>\n            , although much shorter in\n            <jats:italic>M. morganii<\/jats:italic>\n            than in the other enterobacterial species, suggested similar AmpR regulatory properties. The MICs of \u03b2-lactams for\n            <jats:italic>E. coli<\/jats:italic>\n            MC4100 (\n            <jats:italic>ampC<\/jats:italic>\n            mutant) harboring recombinant plasmid pACYC184 containing either\n            <jats:italic>ampC<\/jats:italic>\n            and\n            <jats:italic>ampR<\/jats:italic>\n            (pAC-1) or\n            <jats:italic>ampC<\/jats:italic>\n            (pAC-2) and induction experiments showed that the\n            <jats:italic>ampC<\/jats:italic>\n            gene of\n            <jats:italic>M. morganii<\/jats:italic>\n            1 was repressed in the presence of\n            <jats:italic>ampR<\/jats:italic>\n            and was activated when a \u03b2-lactam inducer was added. Moreover, transformation of\n            <jats:italic>M. morganii<\/jats:italic>\n            1 or of\n            <jats:italic>E. coli<\/jats:italic>\n            JRG582 (\u0394\n            <jats:italic>ampDE<\/jats:italic>\n            ) harboring\n            <jats:italic>ampC<\/jats:italic>\n            and\n            <jats:italic>ampR<\/jats:italic>\n            with a recombinant plasmid containing\n            <jats:italic>ampD<\/jats:italic>\n            from\n            <jats:italic>E. cloacae<\/jats:italic>\n            resulted in a decrease in the \u03b2-lactam MICs and an inducible phenotype for\n            <jats:italic>M. morganii<\/jats:italic>\n            1, thus underlining the role of an AmpD-like protein in the regulation of the\n            <jats:italic>M. morganii<\/jats:italic>\n            cephalosporinase. Fifteen other\n            <jats:italic>M. morganii<\/jats:italic>\n            clinical isolates with phenotypes of either low-level inducible cephalosporinase expression or high-level constitutive cephalosporinase expression harbored the same\n            <jats:italic>ampC-ampR<\/jats:italic>\n            organization, with the\n            <jats:italic>hybF<\/jats:italic>\n            and\n            <jats:italic>orf-1<\/jats:italic>\n            genes surrounding them; the organization of these genes thus differed from those of\n            <jats:italic>ampC-ampR<\/jats:italic>\n            genes in\n            <jats:italic>C. freundii<\/jats:italic>\n            and\n            <jats:italic>E. cloacae<\/jats:italic>\n            , which are located downstream from the fumarate operon. Finally, an identical AmpC \u03b2-lactamase (DHA-1) was recently identified as being plasmid encoded in\n            <jats:italic>Salmonella enteritidis<\/jats:italic>\n            , and this is confirmatory evidence of a chromosomal origin of the plasmid-mediated cephalosporinases.\n          <\/jats:p>","DOI":"10.1128\/aac.43.4.769","type":"journal-article","created":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T20:34:41Z","timestamp":1539030881000},"page":"769-776","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":99,"title":["Cloning, Sequence Analyses, Expression, and Distribution of\n            <i>ampC-ampR<\/i>\n            from\n            <i>Morganella morganii<\/i>\n            Clinical Isolates"],"prefix":"10.1128","volume":"43","author":[{"given":"Laurent","family":"Poirel","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Guibert","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital Antoine Be\u0301cle\u0300re, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 92141 Clamart,2 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Girlich","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital Antoine Be\u0301cle\u0300re, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 92141 Clamart,2 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/25.2.199"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1980.0049"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(97)00006-2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.9.2352"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.2.153"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.134.3.1141-1156.1978"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1111\/j.1432-1033.1987.tb13362.x","article-title":"Nucleotide sequence and comparative analysis of the frd operon encoding the fumarate reductase of Proteus vulgaris.","volume":"167","author":"Cole S. 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