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Administration of thiolated rhTNF-\u03b1 with protected thiol groups (rhTNF\u03b1-ATA; no stabilized trimers in vitro) exhibited the same protective efficacy against ECM, while in vitro bioactivity was reduced. Parasitemia was significantly suppressed in rhTNF-treated mice that were protected against ECM but not in treated mice that developed ECM. Protection against ECM was not related to increased concentrations in plasma of soluble TNF receptor 1 and 2 directly after injection or at the moment of development of ECM in nontreated mice. The results indicate that thiolation of rhTNF-\u03b1 leads to the formation of stable trimers with increased potential in vivo.<\/jats:p>","DOI":"10.1128\/aac.43.5.1027","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:34:57Z","timestamp":1539045297000},"page":"1027-1033","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Thiolated Recombinant Human Tumor Necrosis Factor-Alpha Protects against<i>Plasmodium berghei<\/i>K173-Induced Experimental Cerebral Malaria in Mice"],"prefix":"10.1128","volume":"43","author":[{"given":"Nancy S.","family":"Postma","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3508 TB Utrecht,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob C.","family":"Hermsen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Medical Microbiology, University Hospital Nijmegen, 6500 HB Nijmegen,2 The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daan J. 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