{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:46:01Z","timestamp":1792025161381,"version":"4.4.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Production of a metallo-\u03b2-lactamase activity was detected in a carbapenem-resistant\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    clinical isolate (isolate VR-143\/97) from an Italian inpatient at the Verona University Hospital (northern Italy). The metallo-\u03b2-lactamase determinant was isolated from a genomic library of VR-143\/97, constructed in an\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    plasmid vector, by screening for clones with reduced susceptibility to imipenem. Sequencing of the cloned gene revealed that it encoded a new class B \u03b2-lactamase that was named VIM-1. At the sequence level VIM-1 was rather divergent from the other class B enzymes (16.4 to 38.7% identity), overall being more similar to members of subclass B1 including the \u03b2-lactamase II of\n                    <jats:italic>Bacillus cereus<\/jats:italic>\n                    (Bc-II), the\n                    <jats:italic>Bacteroides fragilis<\/jats:italic>\n                    CcrA, the\n                    <jats:italic>Chryseobacterium meningosepticum<\/jats:italic>\n                    BlaB, and the cassette-encoded IMP-1 enzymes. Among these, VIM-1 showed the highest degree of similarity to Bc-II. Similarly to\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>IMP<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>VIM<\/jats:sub>\n                    was also found to be carried on a gene cassette inserted into a class 1 integron. The\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>VIM<\/jats:sub>\n                    -containing integron was located on the chromosome of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    VR-143\/97, and the metallo-\u03b2-lactamase-encoding determinant was not transferable to\n                    <jats:italic>E. coli<\/jats:italic>\n                    by conjugation. Expression of the integron-borne\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>VIM<\/jats:sub>\n                    gene in\n                    <jats:italic>E. coli<\/jats:italic>\n                    resulted in a significant decrease in susceptibility to a broad array of \u03b2-lactams (ampicillin, carbenicillin, piperacillin, mezlocillin, cefotaxime, cefoxitin, ceftazidime, cefoperazone, cefepime, and carbapenems), revealing a very broad substrate specificity of the VIM-1 enzyme.\n                  <\/jats:p>","DOI":"10.1128\/aac.43.7.1584","type":"journal-article","created":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T20:35:16Z","timestamp":1539030916000},"page":"1584-1590","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":570,"title":["Cloning and Characterization of\n                    <i>bla<\/i>\n                    <sub>VIM<\/sub>\n                    , a New Integron-Borne Metallo-\u03b2-Lactamase Gene from a\n                    <i>Pseudomonas aeruginosa<\/i>\n                    Clinical Isolate"],"prefix":"10.1128","volume":"43","author":[{"given":"Laura","family":"Lauretti","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, 53100-Siena,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Letizia","family":"Riccio","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, 53100-Siena,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annarita","family":"Mazzariol","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Istituto di Microbiologia, Universita\u0300 di Verona, 37134-Verona,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Cornaglia","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Istituto di Microbiologia, Universita\u0300 di Verona, 37134-Verona,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Amicosante","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Dipartimento di Scienze e Tecnologie Biomediche e Biometria, Universita\u0300 dell\u2019Aquila, 67100-L\u2019Aquila,3 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Fontana","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Istituto di Microbiologia, Universita\u0300 di Verona, 37134-Verona,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, 53100-Siena,1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.7.1612"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/514922"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00174.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.134.3.1141-1156.1978"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/S0969-2126(96)00089-5","article-title":"Crystal structure of the wide-spectrum binuclear zinc \u03b2-lactamase from Bacteroides fragilis.","volume":"4","author":"Concha N.","year":"1996","unstructured":"Concha N. 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