{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T14:52:45Z","timestamp":1774795965117,"version":"3.50.1"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A clinical isolate of\n            <jats:italic>Klebsiella oxytoca<\/jats:italic>\n            (Kox 443) was found to have a low-level resistance to broad-spectrum penicillins (MICs of amoxicillin and ticarcillin, 256 and 32 \u03bcg\/ml, respectively), without substantial potentiation by 2 \u03bcg of clavulanic acid per ml (amoxicillin- and ticarcillin-clavulanate, 128 and 8 \u03bcg\/ml, respectively), while being fully susceptible to cephalosporins and other \u03b2-lactam antibiotics. These resistances were carried by a ca. 50-kb conjugative plasmid that encodes a single \u03b2-lactamase with a pI of 5.6. Compared to TEM-2, this enzyme exhibited a 3- to 30-fold higher\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            and a decreased maximal hydrolysis rate for \u03b2-lactams; higher concentrations of suicide inactivators (5- to 500-fold higher concentrations giving a 50% reduction in hydrolysis) were required for inhibition. Nucleotide sequence analysis revealed identity between the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            gene of Kox 443 and the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-2<\/jats:sub>\n            gene, except for a single A-to-G change at position 590, leading to the amino acid change from Ser-130 Gly. This mutation has not been reported previously in the TEM type \u03b2-lactamases produced by clinical strains, and the novel enzyme was called TEM-59 (alternative name IRT-17). This is the first description of an inhibitor-resistant TEM-derived enzyme in the species\n            <jats:italic>K. oxytoca.<\/jats:italic>\n          <\/jats:p>","DOI":"10.1128\/aac.43.7.1657","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T00:35:16Z","timestamp":1539045316000},"page":"1657-1661","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Molecular Characterization of TEM-59 (IRT-17), a Novel Inhibitor-Resistant TEM-Derived \u03b2-Lactamase in a Clinical Isolate of\n            <i>Klebsiella oxytoca<\/i>"],"prefix":"10.1128","volume":"43","author":[{"given":"H.","family":"Bermudes","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Microbiologie, UFR des Sciences Pharmaceutiques, Universite\u0301 de Bordeaux 2,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Jude","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Microbiologie, UFR des Sciences Pharmaceutiques, Universite\u0301 de Bordeaux 2,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. B.","family":"Chaibi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->UMR 175 CNRS-MNHN, 29000 Quimper,2 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Arpin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Microbiologie, UFR des Sciences Pharmaceutiques, Universite\u0301 de Bordeaux 2,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Bebear","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Laboratoire de Bacte\u0301riologie, Ho\u0302pital Pellegrin,333076 Bordeaux Cedex, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Labia","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->UMR 175 CNRS-MNHN, 29000 Quimper,2 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Quentin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Microbiologie, UFR des Sciences Pharmaceutiques, Universite\u0301 de Bordeaux 2,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.33.1.63"},{"key":"e_1_3_1_4_2","first-page":"75","article-title":"Nucleotide sequences of the genes coding for the TEM-like \u03b2-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2).","volume":"120","author":"Belaaouaj A.","year":"1994","unstructured":"Belaaouaj A. Lapoumeroulie C. Cani\u00e7a M. M. Vedel G. N\u00e9vot P. Krishnamoorthy R. Paul G. Nucleotide sequences of the genes coding for the TEM-like \u03b2-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2). FEMS Microbiol. Lett. 120 1994 75 80","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.1.222"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.10.2059"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.11.2547"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/38.2.183"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi961044g"},{"key":"e_1_3_1_10_2","first-page":"111","article-title":"Characterization and amino acid sequence of IRT-4, a novel TEM-type enzyme with a decreased susceptibility to \u03b2-lactamase inhibitors.","volume":"120","author":"Brun T.","year":"1994","unstructured":"Brun T. P\u00e9duzzi J. Cani\u00e7a M. M. Paul G. N\u00e9vot P. Barth\u00e9l\u00e9my M. Labia R. Characterization and amino acid sequence of IRT-4, a novel TEM-type enzyme with a decreased susceptibility to \u03b2-lactamase inhibitors. FEMS Microbiol. Lett. 120 1994 111 118","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/39.1.1"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.2.374"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00006121"},{"key":"e_1_3_1_14_2","first-page":"343","article-title":"D\u00e9termination du point iso\u00e9lectrique des \u03b2-lactamases extraites de 67 souches de Klebsiella oxytoca et comportement ph\u00e9notypique vis-\u00e0-vis de huit \u03b2-lactamines.","volume":"41","author":"Chardon H.","year":"1993","unstructured":"Chardon H. Pachetti C. Collet L. Bellon O. Lagier E. 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