{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:03:38Z","timestamp":1791237818112,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[1999,8,1]],"date-time":"1999-08-01T00:00:00Z","timestamp":933465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[1999,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            strain resistant to oxyimino cephalosporins was cultured from respiratory secretions of a patient suffering from nosocomial pneumonia in Kiel, Germany, in 1997. The isolate harbors a\n            <jats:italic>bla<\/jats:italic>\n            resistance gene located on a transmissible plasmid. An\n            <jats:italic>Escherichia coli<\/jats:italic>\n            transconjugant produces a \u03b2-lactamase with an isoelectric point of 7.7 and a resistance phenotype characteristic of an AmpC (class 1) \u03b2-lactamase except for low MICs of cephamycins. The\n            <jats:italic>bla<\/jats:italic>\n            gene was cloned and sequenced. It encodes a protein of 386 amino acids with the active site serine of the S-X-X-K motif at position 64, as is characteristic for class C \u03b2-lactamases. Multiple alignment of the deduced amino acid sequence with 21 other AmpC \u03b2-lactamases demonstrates only very distant homology, reaching at maximum 52.3% identity for the chromosomal AmpC \u03b2-lactamase of\n            <jats:italic>Serratia marcescens<\/jats:italic>\n            SR50. The \u03b2-lactamase of\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            KUS represents a new type of AmpC-class enzyme, for which we propose the designation ACC-1 (Ambler class C-1).\n          <\/jats:p>","DOI":"10.1128\/aac.43.8.1924","type":"journal-article","created":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T20:35:20Z","timestamp":1539030920000},"page":"1924-1931","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":96,"title":["A Novel Type of AmpC \u03b2-Lactamase, ACC-1, Produced by a\n            <i>Klebsiella pneumoniae<\/i>\n            Strain Causing Nosocomial Pneumonia"],"prefix":"10.1128","volume":"43","author":[{"given":"Adolf","family":"Bauernfeind","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Max von Pettenkofer Institute, Munich,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Schneider","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Max von Pettenkofer Institute, Munich,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renate","family":"Jungwirth","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Max von Pettenkofer Institute, Munich,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany","family":"Sahly","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institute for Medical Microbiology and Virology, Kiel,2 Federal Republic of Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"Ullmann","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institute for Medical Microbiology and Virology, Kiel,2 Federal Republic of Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(97)00006-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.9.2352"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"520","DOI":"10.3349\/ymj.1998.39.6.520","article-title":"Plasmid-encoded AmpC \u03b2-lactamases: how far have we gone 10 years after their discovery?","volume":"39","author":"Bauernfeind A.","year":"1998","unstructured":"Bauernfeind A. Chong Y. Lee K. Plasmid-encoded AmpC \u03b2-lactamases: how far have we gone 10 years after their discovery? Yonsei Med. J. 39 1998 520 525","journal-title":"Yonsei Med. J."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1007\/BF01650718","article-title":"Extended broad spectrum \u03b2-lactamase in Klebsiella pneumoniae including resistance to cephamycins.","volume":"17","author":"Bauernfeind A.","year":"1989","unstructured":"Bauernfeind A. Chong Y. Schweighart S. Extended broad spectrum \u03b2-lactamase in Klebsiella pneumoniae including resistance to cephamycins. Infection 17 1989 316 321","journal-title":"Infection"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01647010"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.1.221"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.8.1926"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.9.2041"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_3_2_11_2","unstructured":"Boyer-Mariotte\n              S.\n              Raskine\n              L.\n              Hanau\n              B.\n              Philippon\n              A.\n              Sanson-Le Pors\n              M. J.\n              Arlet\n              G.\n          Novel plasmid-mediated AmpC-type \u03b2-lactamase (MOX-2) in a clinical isolate of Klebsiella pneumoniae abstr. C7\n          Abstracts of the 38th Interscience Conference on Antimicrobial Agents and Chemotherapy.\n          1998\n          70\n          American Society for Microbiology\n          Washington D.C"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.3.563"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/39.1.1"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.33.9.1580"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/208239a0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.5.1182"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1042\/bj2500753","article-title":"Sequence analysis of three Enterobacter cloacae ampC \u03b2-lactamase genes and their products.","volume":"250","author":"Galleni M.","year":"1988","unstructured":"Galleni M. 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