{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T07:53:26Z","timestamp":1768463606928,"version":"3.49.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In vitro synergy between extended-spectrum cephalosporins and either clavulanic acid or cefoxitin was found for\n            <jats:italic>Chryseobacterium meningosepticum<\/jats:italic>\n            isolates during a double-disk assay on an agar plate. An extended-spectrum \u03b2-lactamase (ESBL) gene from a\n            <jats:italic>C. meningosepticum<\/jats:italic>\n            clinical isolate was cloned and expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            DH10B. Its protein conferred resistance to most \u03b2-lactams including extended-spectrum cephalosporins but not to cephamycins or to imipenem. Its activity was strongly inhibited by clavulanic acid, sulbactam, and tazobactam, as well as by cephamycins and imipenem. Sequence analysis of the cloned DNA fragment revealed an open reading frame (ORF) of 891 bp with a G+C content of 33.9%, which lies close to the expected range of G+C contents of members of the\n            <jats:italic>Chryseobacterium<\/jats:italic>\n            genus. The ORF encoded a precursor protein of 297 amino acids, giving a mature protein with a molecular mass of 31 kDa and a pI value of 9.2 in\n            <jats:italic>E. coli<\/jats:italic>\n            . This gene was very likely chromosomally located. Amino acid sequence comparison showed that this \u03b2-lactamase, named CME-2 (\n            <jats:italic>C. meningosepticum<\/jats:italic>\n            ESBL), is a novel ESBL of the Ambler class A group (Bush functional group 2be), being weakly related to other class A \u03b2-lactamases. It shares only 39 and 35% identities with the ESBLs VEB-1 from\n            <jats:italic>E. coli<\/jats:italic>\n            MG-1 and CBL-A from\n            <jats:italic>Bacteroides uniformis<\/jats:italic>\n            , respectively. The distribution of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CME-2<\/jats:sub>\n            among unrelated\n            <jats:italic>C. meningosepticum<\/jats:italic>\n            species isolates showed that\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CME-2<\/jats:sub>\n            -like genes were found in the\n            <jats:italic>C. meningosepticum<\/jats:italic>\n            strains studied but were absent from strains of other\n            <jats:italic>C. meningosepticum<\/jats:italic>\n            -related species. Each\n            <jats:italic>C. meningosepticum<\/jats:italic>\n            strain produced at least two \u03b2-lactamases, with one of them being a noninducible serine ESBL with variable pIs ranging from 7.0 to 8.5.\n          <\/jats:p>","DOI":"10.1128\/aac.44.1.1-9.2000","type":"journal-article","created":{"date-parts":[[2009,4,22]],"date-time":"2009-04-22T05:03:57Z","timestamp":1240376637000},"page":"1-9","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Genetic-Biochemical Analysis and Distribution of the Ambler Class A \u03b2-Lactamase CME-2, Responsible for Extended-Spectrum Cephalosporin Resistance in\n            <i>Chryseobacterium<\/i>\n            (\n            <i>Flavobacterium<\/i>\n            )\n            <i>meningosepticum<\/i>"],"prefix":"10.1128","volume":"44","author":[{"given":"Samuel","family":"Bellais","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delphine","family":"Girlich","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1980.0049"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(92)90121-S"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1097\/00005792-199701000-00003","article-title":"Chryseobacterium meningosepticum: an emerging pathogen among immunocompromised adults. 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