{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T09:43:26Z","timestamp":1784195006143,"version":"3.55.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2000,10,1]],"date-time":"2000-10-01T00:00:00Z","timestamp":970358400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A murine model of intratracheally induced histoplasmosis in immunocompromised B6C3F<jats:sub>1<\/jats:sub>mice was used to evaluate a new triazole antifungal agent, posaconazole. This compound was previously shown to be comparable to amphotericin B and superior to itraconazole for the treatment of histoplasmosis in immunocompetent mice. The current study used mice that were depleted of T lymphocytes by intraperitoneal injection of anti-CD4 and anti-CD8 monoclonal antibodies beginning 2 days before infection and continuing at 5-day intervals until completion of the study. Groups of B6C3F<jats:sub>1<\/jats:sub>mice that were depleted of CD4 and CD8 T cells were infected with an inoculum of 10<jats:sup>4<\/jats:sup><jats:italic>Histoplasma capsulatum<\/jats:italic>yeasts. All mice receiving posaconazole at 1 or 0.1 mg\/kg of body weight\/day, amphotericin B at 2 mg\/kg every other day (qod), or itraconazole at 75 mg\/kg\/day survived to day 29. Only 60% of mice receiving itraconazole at 10 mg\/kg\/day and none receiving amphotericin B at 0.2 mg\/kg qod survived to that date. Fungal burdens were determined at day 14 of infection, 1 day after discontinuation of therapy. Quantitative colony counts and<jats:italic>Histoplasma<\/jats:italic>antigen levels in lung and spleen tissues declined following treatment with amphotericin B at 2 mg\/kg qod, posaconazole at 5 and 1 mg\/kg\/day, and itraconazole at 75 mg\/kg\/day but not in mice treated with amphotericin B at 0.2 mg\/kg qod or itraconazole at 10 mg\/kg\/day. Posaconazole at 0.1 mg\/kg\/day reduced fungal colony counts and antigen levels in spleens but not in lungs. This study shows posaconazole activity for the treatment of histoplasmosis in immunosuppressed animals.<\/jats:p>","DOI":"10.1128\/aac.44.10.2604-2608.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"2604-2608","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Comparison of a New Triazole, Posaconazole, with Itraconazole and Amphotericin B for Treatment of Histoplasmosis following Pulmonary Challenge in Immunocompromised Mice"],"prefix":"10.1128","volume":"44","author":[{"given":"Patricia","family":"Connolly","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Medicine1 and"},{"name":"<!--label omitted: 2-->Histoplasmosis Reference Laboratory,2 Indianapolis, Indiana, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Joseph","family":"Wheat","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medicine1 and"},{"name":"<!--label omitted: 3-->Department of Pathology and Laboratory Medicine,3 Indiana University School of Medicine,"},{"name":"<!--label omitted: 4-->Department of Veterans' Affairs Hospital,4 and"},{"name":"<!--label omitted: 2-->Histoplasmosis Reference Laboratory,2 Indianapolis, Indiana, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Schnizlein-Bick","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medicine1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Durkin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medicine1 and"},{"name":"<!--label omitted: 2-->Histoplasmosis Reference Laboratory,2 Indianapolis, Indiana, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Kohler","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Department of Veterans' Affairs Hospital,4 and"},{"name":"<!--label omitted: 2-->Histoplasmosis Reference Laboratory,2 Indianapolis, Indiana, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda","family":"Smedema","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medicine1 and"},{"name":"<!--label omitted: 2-->Histoplasmosis Reference Laboratory,2 Indianapolis, Indiana, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet","family":"Goldberg","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medicine1 and"},{"name":"<!--label omitted: 2-->Histoplasmosis Reference Laboratory,2 Indianapolis, Indiana, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Brizendine","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medicine1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Loebenberg","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Schering-Plough Research Institute, Kenilworth, New Jersey5"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.100.3.1271-1276.1969"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.7.2564-2569.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.2.322"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1080\/00031305.1981.10479327","article-title":"Rank transformations as a bridge between parametric and nonparametric statistics.","volume":"35","author":"Conover W. 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