{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:00:35Z","timestamp":1790686835819,"version":"4.1.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2000,11,1]],"date-time":"2000-11-01T00:00:00Z","timestamp":973036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Serratia marcescens<\/jats:italic>\n            Rio-5, one of 18 extended-spectrum \u03b2-lactamase (ESBL)-producing strains isolated in several hospitals in Rio de Janeiro (Brazil) in 1996 and 1997, exhibited a high level of resistance to aztreonam (MIC, 512 \u03bcg\/ml) and a distinctly higher level of resistance to cefotaxime (MIC, 64 \u03bcg\/ml) than to ceftazidime (MIC, 8 \u03bcg\/ml). The strain produced a plasmid-encoded ESBL with a pI of 7.5 whose\n            <jats:italic>bla<\/jats:italic>\n            gene was not related to those of other plasmid-mediated Ambler class A ESBLs. Cloning and sequencing revealed a\n            <jats:italic>bla<\/jats:italic>\n            gene encoding a novel class A \u03b2-lactamase in functional group 2be, designated BES-1 (Brazil extended-spectrum \u03b2-lactamase). This enzyme had 51% identity with chromosomal class A penicillinase of\n            <jats:italic>Yersinia enterocolitica<\/jats:italic>\n            Y56, which was the most closely related enzyme and 47 to 48% identity with CTX-M-type \u03b2-lactamases, which were the most closely related ESBLs. In common with CTX-M enzymes, BES-1 exhibited high cefotaxime-hydrolyzing activity (\n            <jats:italic>k<\/jats:italic>\n            <jats:sub>cat<\/jats:sub>\n            , 425 s\n            <jats:sup>\u22121<\/jats:sup>\n            ). However, BES-1 differed from CTX-M enzymes by its significant ceftazidime-hydrolyzing activity (\n            <jats:italic>k<\/jats:italic>\n            <jats:sub>cat<\/jats:sub>\n            , 25 s\n            <jats:sup>\u22121<\/jats:sup>\n            ), high affinity for aztreonam (\n            <jats:italic>\n              K\n              <jats:sub>i<\/jats:sub>\n            <\/jats:italic>\n            , 1 \u03bcM), and lower susceptibility to tazobactam (50% inhibitory concentration [IC\n            <jats:sub>50<\/jats:sub>\n            ], 0.820 \u03bcM) than to clavulanate (IC\n            <jats:sub>50<\/jats:sub>\n            , 0.045 \u03bcM). Likewise, certain characteristic structural features of CTX-M enzymes, such as Phe-160, Ser-237, and Arg-276, were observed for BES-1, which, in addition, harbored different residues (Ala-104, Ser-171, Arg-220, Gly-240) and six additional residues at the end of the sequence. BES-1, therefore, may be an interesting model for further investigations of the structure-function relationships of class A ESBLs.\n          <\/jats:p>","DOI":"10.1128\/aac.44.11.3061-3068.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"3061-3068","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":84,"title":["A Novel Class A Extended-Spectrum \u03b2-Lactamase (BES-1) in\n            <i>Serratia marcescens<\/i>\n            Isolated in Brazil"],"prefix":"10.1128","volume":"44","author":[{"given":"R.","family":"Bonnet","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L. M.","family":"Sampaio","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Setor de Bacteriologia, Laborato\u0301rio La\u0302mina LTDA, 71-Botafogo, Rio de Janeiro, RJ, Brazil 22280-0302"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Chanal","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Sirot","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"De Champs","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"Viallard","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Laboratoire de Biochimie,3 Faculte\u0301 de Me\u0301decine, 63001 Clermont-Ferrand Cedex, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Labia","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->CNRS-UBO-MNHN, FRE 2125, 29000 Quimper,4 France, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Sirot","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.33.1.63"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(92)90121-S"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.2.509"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.3.616"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)40181-6"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1936-1942.2000"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3301443"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/12.4.313"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.8.1980"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(97)00127-1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/35.2.281"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2450911"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.7.1444"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13331.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.5.1259"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2432"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.10.2269"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/4.7.811"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.4.867"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.11.2294"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.28.2.302"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.12.2593"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(73)80154-1"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.15.4611-4617.1991"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.2.307"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.3.629"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.16.7693"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/10.1.1"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.1.104"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/44.4.489"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.33.12.2160"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(97)00020-4"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(94)90048-5"},{"key":"e_1_3_2_38_2","first-page":"79","article-title":"Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus.","volume":"67","author":"Perilli M. 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