{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:13:22Z","timestamp":1790003602491,"version":"4.0.1"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2000,11,1]],"date-time":"2000-11-01T00:00:00Z","timestamp":973036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Acidaminococcus fermentans<\/jats:italic>\n            belongs to the group of strictly anaerobic gram-negative cocci. All previously described\n            <jats:italic>Acidaminococcus<\/jats:italic>\n            strains are susceptible to \u03b2-lactam antibiotics. An\n            <jats:italic>A. fermentans<\/jats:italic>\n            strain (RYC-MR95) resistant to penicillin and expanded-spectrum cephalosporin (amoxicillin and cefotaxime MICs, 64 \u03bcg\/ml) was isolated from a human perianal abscess. A fragment encoding a \u03b2-lactamase from genomic DNA was cloned in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            K-12 strain HB101, and the recombinant strain expressed resistance to amoxicillin (MIC, 1,024 \u03bcg\/ml) and cefotaxime (MIC, 4 \u03bcg\/ml). Clavulanic acid decreased the MICs to 8 and 0.03 \u03bcg\/ml, respectively. Analysis of the nucleotide sequence revealed a new class A \u03b2-lactamase, ACI-1. In accordance with its biochemical properties, we propose to assign ACI-1 to functional group 2be. The ACI-1 enzyme (estimated pI 4.3) had &lt;50% amino acid identity with any other class A \u03b2-lactamases, the closest being ROB-1 from\n            <jats:italic>Haemophilus influenzae<\/jats:italic>\n            (44%). ACI-1 was closer to class A \u03b2-lactamases from some gram-positive organisms (41 to 44% amino acid identity with\n            <jats:italic>Bacillus<\/jats:italic>\n            \u03b2-lactamases) than to most class A enzymes from gram-negative organisms (TEM-1, 24.6%). The\n            <jats:italic>aci1<\/jats:italic>\n            gene had a G+C content of 42.1%, in contrast with 56% G+C content for genomic DNA from\n            <jats:italic>A. fermentans<\/jats:italic>\n            , thus suggesting that\n            <jats:italic>aci1<\/jats:italic>\n            may have been obtained by horizontal gene transfer.\n          <\/jats:p>","DOI":"10.1128\/aac.44.11.3144-3149.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"3144-3149","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["ACI-1 from\n            <i>Acidaminococcus fermentans<\/i>\n            : Characterization of the First \u03b2-Lactamase in Anaerobic Cocci"],"prefix":"10.1128","volume":"44","author":[{"given":"J. C.","family":"Gala\u0301n","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Servicio de Microbiolog\u0131\u0301a, Hospital Ramo\u0301n y Cajal, Instituto Nacional de Salud (INSALUD), 28034 Madrid,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Reig","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Servicio de Microbiolog\u0131\u0301a, Hospital Ramo\u0301n y Cajal, Instituto Nacional de Salud (INSALUD), 28034 Madrid,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Navas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Museo Nacional de Ciencias Naturales, CSIC, 28006 Madrid,2 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Baquero","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Servicio de Microbiolog\u0131\u0301a, Hospital Ramo\u0301n y Cajal, Instituto Nacional de Salud (INSALUD), 28034 Madrid,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Bla\u0301zquez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Servicio de Microbiolog\u0131\u0301a, Hospital Ramo\u0301n y Cajal, Instituto Nacional de Salud (INSALUD), 28034 Madrid,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1993.tb00348.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/33.1.33"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.10.2059"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(69)90288-5"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.134.3.1141-1156.1978"},{"key":"e_1_3_2_10_2","first-page":"333","article-title":"Non-sporing anaerobes in certain surgical group of patients.","volume":"93","author":"Chatterjee B. 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P.\n              Moore\n              W. E. 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F.\n              Maniatis\n              T.\n          Molecular cloning: a laboratory manual\n          2nd ed.\n          1989\n          Cold Spring Harbor Laboratory Press\n          Cold Spring Harbor N.Y"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.8.1711"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(86)90117-4"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.27.6.943"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi981210f"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(85)90120-9"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.44.11.3144-3149.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.44.11.3144-3149.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T16:37:09Z","timestamp":1645461429000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.44.11.3144-3149.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11]]},"references-count":42,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2000,11]]}},"alternative-id":["10.1128\/AAC.44.11.3144-3149.2000"],"URL":"https:\/\/doi.org\/10.1128\/aac.44.11.3144-3149.2000","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,11]]},"assertion":[{"value":"2000-02-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-08-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}