{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:12:52Z","timestamp":1791090772931,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Clinical strains of\n                    <jats:italic>Stenotrophomonas maltophilia<\/jats:italic>\n                    are often highly resistant to multiple antibiotics, although the mechanisms of resistance are generally poorly understood. Multidrug resistant (MDR) strains were readily selected by plating a sensitive reference strain of the organism individually onto a variety of antibiotics, including tetracycline, chloramphenicol, ciprofloxacin, and norfloxacin. Tetracycline-selected MDR strains typically showed cross-resistance to erythromycin and fluoroquinolones and, in some instances, aminoglycosides. MDR mutants selected with the other agents generally displayed resistance to chloramphenicol and fluoroquinolones only, although two MDR strains (e.g., K1385) were also resistant to erythromycin and hypersusceptible to aminoglycosides. Many of the MDR strains expressed either moderate or high levels of a novel outer membrane protein (OMP) of ca. 50 kDa molecular mass, a phenotype typical of MDR strains of\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    hyperexpressing drug efflux systems. Indeed, the 50-kDa OMP of these\n                    <jats:italic>S. maltophilia<\/jats:italic>\n                    MDR strains reacted with antibody to OprM, the outer membrane component of the MexAB-OprM MDR efflux system of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    . Similarly, a ca. 110-kDa cytoplasmic membrane protein of these MDR strains also reacted with antibody to the MexB component of the\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    pump. The outer and cytoplasmic membranes of several clinical\n                    <jats:italic>S. maltophilia<\/jats:italic>\n                    strains also reacted with the anti-OprM and anti-MexB antibodies. N-terminal amino acid sequencing of a cyanogen bromide-generated peptide of the 50-kDa OMP of MDR strain K1385, dubbed SmeM (\n                    <jats:italic>Stenotrophomonas<\/jats:italic>\n                    multidrug efflux), revealed it to be very similar to a number of outer membrane multidrug efflux components of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas putida<\/jats:italic>\n                    . Deletion of the L1 and L2 \u03b2-lactamase genes confirmed that these enzymes were responsible for the bulk of the \u03b2-lactam resistance of K1385 and its parent. Still, overexpression of the MDR efflux mechanism in an L1- and L2-deficient derivative of K1385 did yield a modest increase in resistance to a few \u03b2-lactams. These data are consistent with the MDR efflux mechanism(s) playing a role in the multidrug resistance of\n                    <jats:italic>S. maltophilia<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/aac.44.2.287-293.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:56:57Z","timestamp":1027749417000},"page":"287-293","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":152,"title":["Multiple Antibiotic Resistance in\n                    <i>Stenotrophomonas maltophilia<\/i>\n                    : Involvement of a Multidrug Efflux System"],"prefix":"10.1128","volume":"44","author":[{"given":"Li","family":"Zhang","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian-Zhi","family":"Li","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Poole","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.5.1140"},{"key":"e_1_3_2_3_2","unstructured":"Ausubel\n              F. 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