{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T13:04:17Z","timestamp":1773752657907,"version":"3.50.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Twenty (8.5%) of 234 nonrepetitive clinical isolates of\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            from southern Taiwan were found to produce extended-spectrum \u03b2-lactamases (ESBLs): 10 strains produced SHV-12, 4 produced SHV-5, 2 produced a non-TEM non-SHV ESBL with a pI of 8.3, 3 produced a novel AmpC \u03b2-lactamase designated CMY-8 with a pI of 8.25, and 1 produced SHV-12 and an unidentified AmpC enzyme with a pI of 8.2. The CMY-8 enzyme confers a resistance phenotype similar to CMY-1 and MOX-1, and sequence comparisons showed high homologies (&gt;95%) of nucleotide and amino acid sequences among these three enzymes. Plasmid and pulse-field gel electrophoresis analyses revealed that all isolates harboring an SHV-derived ESBL were genetically unrelated, indicating that dissemination of resistance plasmids is responsible for the spread of SHV ESBLs among\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            in this area. All three isolates carrying CMY-8 had identical genotypic patterns, suggesting the presence of an epidemic strain.\n          <\/jats:p>","DOI":"10.1128\/aac.44.6.1438-1442.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"1438-1442","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":84,"title":["Prevalence of SHV-12 among Clinical Isolates of\n            <i>Klebsiella pneumoniae<\/i>\n            Producing Extended-Spectrum \u03b2-Lactamases and Identification of a Novel AmpC Enzyme (CMY-8) in Southern Taiwan"],"prefix":"10.1128","volume":"44","author":[{"given":"Jing-Jou","family":"Yan","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology,1 National Cheng Kung University Medical Center, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiou-Mei","family":"Wu","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Medical Technology,2 National Cheng Kung University Medical College, Tainan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Huei","family":"Tsai","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology,1 National Cheng Kung University Medical Center, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiunn-Jong","family":"Wu","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Medical Technology,2 National Cheng Kung University Medical College, Tainan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ih-Jen","family":"Su","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology,1 National Cheng Kung University Medical Center, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.10.2553-2558.1994"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1128\/mr.44.1.1-56.1980","article-title":"Linkage map of Escherichia coli K-12, edition 6.","volume":"44","author":"Bachmann B. J.","year":"1980","unstructured":"Bachmann B. J. Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol. Rev. 44 1980 1451 1456","journal-title":"Microbiol. Rev."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.1.221"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.8.1926"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01647010"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3538420"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.4.785"},{"key":"e_1_3_2_9_2","unstructured":"Farmer\n              J. J.\n              III. Enterobacteriaceae : introduction and identification\n          Manual of clinical microbiology.\n              Murray\n              P. R.\n              Baron\n              E. J.\n              Pfaller\n              M. A.\n              Tenover\n              F. C.\n              Yolken\n              R. 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Gene 139 1994 93 98","journal-title":"Gene"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.4.908-911.1996"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.7.1759"},{"key":"e_1_3_2_13_2","first-page":"661","article-title":"Antimicrobial susceptibility testing for Klebsiella pneumoniae isolates resistant to extended-spectrum \u03b2-lactam antibiotics.","volume":"97","author":"Jan I. S.","year":"1998","unstructured":"Jan I. S. Hsueh P. R. Teng L. J. Ho S. W. Luh K. T. Antimicrobial susceptibility testing for Klebsiella pneumoniae isolates resistant to extended-spectrum \u03b2-lactam antibiotics. J. Formos. Med. Assoc. 97 1998 661 666","journal-title":"J. Formos. Med. 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R.\n          1994\n          319\n          347\n          American Society for Microbiology\n          Washington D.C."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.3.647"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.8.3737"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.20.4.608-613.1984"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.9.2207"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.44.6.1438-1442.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.44.6.1438-1442.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T16:47:26Z","timestamp":1645462046000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.44.6.1438-1442.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,6]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,6]]}},"alternative-id":["10.1128\/AAC.44.6.1438-1442.2000"],"URL":"https:\/\/doi.org\/10.1128\/aac.44.6.1438-1442.2000","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,6]]},"assertion":[{"value":"1999-10-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-02-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}