{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:47:03Z","timestamp":1791150423690,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A naturally occurring AmpC \u03b2-lactamase (cephalosporinase) gene was cloned from the\n            <jats:italic>Hafnia alvei<\/jats:italic>\n            1 clinical isolate and expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . The deduced AmpC \u03b2-lactamase (ACC-2) had a pI of 8 and a relative molecular mass of 37 kDa and showed 50 and 47% amino acid identity with the chromosome-encoded AmpCs from\n            <jats:italic>Serratia marcescens<\/jats:italic>\n            and\n            <jats:italic>Providentia stuartii<\/jats:italic>\n            , respectively. It had 94% amino acid identity with the recently described plasmid-borne cephalosporinase ACC-1 from\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            , suggesting the chromosomal origin of ACC-1. The hydrolysis constants (\n            <jats:italic>k<\/jats:italic>\n            <jats:sub>cat<\/jats:sub>\n            and\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            ) showed that ACC-2 was a peculiar cephalosporinase, since it significantly hydrolyzed cefpirome. Once its gene was cloned and expressed in\n            <jats:italic>E. coli<\/jats:italic>\n            (pDEL-1), ACC-2 conferred resistance to ceftazidime and cefotaxime but also an uncommon reduced susceptibility to cefpirome. A divergently transcribed\n            <jats:italic>ampR<\/jats:italic>\n            gene with an overlapping promoter compared with\n            <jats:italic>ampC<\/jats:italic>\n            (\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>ACC-2<\/jats:sub>\n            ) was identified in\n            <jats:italic>H. alvei<\/jats:italic>\n            1, encoding an AmpR protein that shared 64% amino acid identity with the closest AmpR protein from\n            <jats:italic>P. stuartii<\/jats:italic>\n            . \u03b2-Lactamase induction experiments showed that the\n            <jats:italic>ampC<\/jats:italic>\n            gene was repressed in the absence of\n            <jats:italic>ampR<\/jats:italic>\n            and was activated when cefoxitin or imipenem was added as an inducer. From\n            <jats:italic>H. alvei<\/jats:italic>\n            1 cultures that expressed an inducible-cephalosporinase phenotype, several ceftazidime- and cefpirome-cross-resistant\n            <jats:italic>H. alvei<\/jats:italic>\n            1 mutants were obtained upon selection on cefpirome- or ceftazidime-containing plates, and\n            <jats:italic>H. alvei<\/jats:italic>\n            1 DER, a ceftazidime-resistant mutant, stably overproduced cephalosporinase. Transformation of\n            <jats:italic>H. alvei<\/jats:italic>\n            1 DER or\n            <jats:italic>E. coli<\/jats:italic>\n            JRG582 (\n            <jats:italic>ampDE<\/jats:italic>\n            mutant) harboring\n            <jats:italic>ampC<\/jats:italic>\n            and\n            <jats:italic>ampR<\/jats:italic>\n            from\n            <jats:italic>H. alvei<\/jats:italic>\n            1 with a recombinant plasmid containing\n            <jats:italic>ampD<\/jats:italic>\n            from\n            <jats:italic>E. coli<\/jats:italic>\n            resulted in a decrease in the MIC of \u03b2-lactam and recovery of an inducible phenotype for\n            <jats:italic>H. alvei<\/jats:italic>\n            1 DER. Thus, AmpR and AmpD proteins may regulate biosynthesis of the\n            <jats:italic>H. alvei<\/jats:italic>\n            cephalosporinase similarly to other enterobacterial cephalosporinases.\n          <\/jats:p>","DOI":"10.1128\/aac.44.6.1470-1478.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"1470-1478","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Biochemical-Genetic Characterization and Regulation of Expression of an ACC-1-Like Chromosome-Borne Cephalosporinase from\n            <i>Hafnia alvei<\/i>"],"prefix":"10.1128","volume":"44","author":[{"given":"Delphine","family":"Girlich","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Bellais","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Karim","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/25.2.199"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1980.0049"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.9.2352"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1093\/clinids\/24.6.1263","article-title":"Hafnia alvei infection after liver transplantation.","volume":"24","author":"Barry J. 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