{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T01:28:49Z","timestamp":1778722129693,"version":"3.51.4"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            To further understand the role of penicillin-binding protein 2a (PBP 2a) of\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            in penicillin resistance, we confirmed the identity of the protein as PBP 2a. The PBP 2a protein migrated electrophoretically to a position corresponding to that of PBP 2x, PBP 2a, and PBP 2b of\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            and was absent in a\n            <jats:italic>pbp2a<\/jats:italic>\n            insertional mutant of\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            . We found that the affinities of PBP 2a for penicillins were lower than for cephalosporins and a carbapenem. When compared with other\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            PBPs, PBP 2a exhibited lower affinities for \u03b2-lactam antibiotics, especially penicillins. Therefore, PBP 2a is a low-affinity PBP for \u03b2-lactam antibiotics in\n            <jats:italic>S. pneumoniae.<\/jats:italic>\n          <\/jats:p>","DOI":"10.1128\/aac.44.6.1745-1748.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"1745-1748","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Identification and Characterization of the Penicillin-Binding Protein 2a of\n            <i>Streptococcus pneumoniae<\/i>\n            and Its Possible Role in Resistance to \u03b2-Lactam Antibiotics"],"prefix":"10.1128","volume":"44","author":[{"given":"Genshi","family":"Zhao","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy I.","family":"Meier","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joann","family":"Hoskins","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly A.","family":"McAllister","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1042\/bj2700525","article-title":"Chromogenic depsipeptide substrates for \u03b2-lactamases and penicillin-sensitive DD-peptidases.","volume":"270","author":"Adam M.","year":"1990","unstructured":"Adam M. Damblon C. Plaitin B. Christiaens L. Frere J.-M. Chromogenic depsipeptide substrates for \u03b2-lactamases and penicillin-sensitive DD-peptidases. Biochem. J. 270 1990 525 529","journal-title":"Biochem. J."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1111\/j.1574-6968.1995.tb07433.x","article-title":"Genetics of high level penicillin resistance in clinical isolates of Streptococcus pneumoniae.","volume":"126","author":"Barcus V. A.","year":"1995","unstructured":"Barcus V. A. Ghanekar K. Yeo M. Coffey T. J. Dowson C. G. Genetics of high level penicillin resistance in clinical isolates of Streptococcus pneumoniae. FEMS Microbiol. Lett. 126 1995 299 304","journal-title":"FEMS Microbiol. 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I.\n          1992\n          148\n          197\n          Chapman and Hall\n          Glasgow Scotland","DOI":"10.1007\/978-94-011-2928-2_5"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0076-6879(94)44021-2","article-title":"Serine-type D-ala-D-ala peptidases and penicillin-binding proteins.","volume":"244","author":"Granier B.","year":"1994","unstructured":"Granier B. Jamin M. Adam M. Galleni M. Lakaye B. Zorzi W. Grandchamps J. Wilkin J.-M. Fraipont C. Joris B. Duez C. Nguyen-Disteche M. Coyette J. Leyh-Bouille M. Dusart J. Christiaens L. Frere J.-M. Ghuysen J.-M. Serine-type D-ala-D-ala peptidases and penicillin-binding proteins. 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