{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:11:57Z","timestamp":1789654317723,"version":"4.0.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            K6 (ATCC 700603), a clinical isolate, is resistant to ceftazidime and other oxyimino-\u03b2-lactams. A consistent reduction in the MICs of oxyimino-\u03b2-lactams by at least 3 twofold dilutions in the presence of clavulanic acid confirmed the utility of\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            K6 as a quality control strain for extended-spectrum \u03b2-lactamase (ESBL) detection. Isoelectric-focusing analysis of crude lysates of K6 demonstrated a single \u03b2-lactamase with a pI of 7.8 and a substrate profile showing preferential hydrolysis of cefotaxime compared to ceftazidime. PCR analysis of total bacterial DNA from K6 identified the presence of a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            gene. K6 contained two large plasmids with molecular sizes of approximately 160 and 80 kb. Hybridization of plasmid DNA with a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            -specific probe indicated that a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            gene was encoded on the 80-kb plasmid, which was shown to transfer resistance to ceftazidime in conjugal mating experiments with\n            <jats:italic>Escherichia coli<\/jats:italic>\n            HB101. DNA sequencing of this\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            -related gene revealed that it differs from\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-1<\/jats:sub>\n            at nine nucleotides, five of which resulted in amino acid substitutions: Ile to Phe at position 8, Arg to Ser at position 43, Gly to Ala at position 238, and Glu to Lys at position 240. In addition to the production of this novel ESBL, designated SHV-18, analysis of the outer membrane proteins of K6 revealed the loss of the OmpK35 and OmpK37 porins.\n          <\/jats:p>","DOI":"10.1128\/aac.44.9.2382-2388.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"2382-2388","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["Characterization of the Extended-Spectrum \u03b2-Lactamase Reference Strain,\n            <i>Klebsiella pneumoniae<\/i>\n            K6 (ATCC 700603), Which Produces the Novel Enzyme SHV-18"],"prefix":"10.1128","volume":"44","author":[{"given":"J. Kamile","family":"Rasheed","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303331;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J.","family":"Anderson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303331;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hesna","family":"Yigit","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303331;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Marie","family":"Queenan","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->The R. W. Johnson Pharmaceutical Research Institute, Raritan, New Jersey 088692;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Dome\u0301nech-Sa\u0301nchez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->A\u0301rea de Microbiolog\u0131\u0301a, Departamento de Biolog\u0131\u0301a, Universidad de las Islas Baleares, Palma de Mallorca, Spain3;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana M.","family":"Swenson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303331;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"Biddle","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303331;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Jane","family":"Ferraro","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Microbiology Laboratory, Massachusetts General Hospital, Boston, Massachusetts 021144;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George A.","family":"Jacoby","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Edith Nourse Rogers Memorial Veterans Hospital, Bedford, Massachusetts 017305; and"},{"name":"<!--label omitted: 6-->Lahey Clinic, Burlington, Massachusetts 018056"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred C.","family":"Tenover","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303331;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/0014-5793(88)80734-8","article-title":"Single amino acid substitution between SHV-1 \u03b2-lactamase and cefotaxime-hydrolyzing SHV-2 enzyme.","volume":"231","author":"Barth\u00e9l\u00e9my M.","year":"1988","unstructured":"Barth\u00e9l\u00e9my M. P\u00e9duzzi J. Ben Yaghlane H. Labia R. Single amino acid substitution between SHV-1 \u03b2-lactamase and cefotaxime-hydrolyzing SHV-2 enzyme. 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