{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:57:26Z","timestamp":1792173446780,"version":"4.4.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Small, soluble single-domain fragments derived from the unique variable region of dromedary heavy-chain antibodies (VHHs) against enzymes are known to be potent inhibitors. The immunization of dromedaries with the TEM-1 and BcII \u03b2-lactamases has lead to the isolation of such single-domain antibody fragments specifically recognizing and inhibiting those \u03b2-lactamases. Two VHHs were isolated that inhibit TEM-1 and one BcII inhibiting VHH was identified. All inhibitory VHHs were tight-binding inhibitors. The 50% inhibitory concentrations were determined for all inhibitors and they were all in the same range as the enzyme concentration used in the assay. Addition of the VHHs to the TEM-1 \u03b2-lactamase, expressed on the surface of bacteria, leads to a higher ampicillin sensitivity of the bacteria. This innovative strategy could generate multiple potent inhibitors for all types of \u03b2-lactamases.<\/jats:p>","DOI":"10.1128\/aac.45.10.2807-2812.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"2807-2812","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":320,"title":["\u03b2-Lactamase Inhibitors Derived from Single-Domain Antibody Fragments Elicited in the\n            <i>Camelidae<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"Katja E.","family":"Conrath","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Ultrastructure, Vrije Universiteit Brussel, B-1640 St. Genesius Rode,1and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Lauwereys","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Ultrastructure, Vrije Universiteit Brussel, B-1640 St. Genesius Rode,1and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moreno","family":"Galleni","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institut de Chimie, Centre d'Inge\u0301nerie des Prote\u0301ines, Universite\u0301 de Lie\u0300ge, B-4000 Sart-Tilman, Lie\u0300ge,2 Belgium, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre\u0301","family":"Matagne","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institut de Chimie, Centre d'Inge\u0301nerie des Prote\u0301ines, Universite\u0301 de Lie\u0300ge, B-4000 Sart-Tilman, Lie\u0300ge,2 Belgium, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Fre\u0300re","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institut de Chimie, Centre d'Inge\u0301nerie des Prote\u0301ines, Universite\u0301 de Lie\u0300ge, B-4000 Sart-Tilman, Lie\u0300ge,2 Belgium, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u0308rg","family":"Kinne","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Central Veterinary Research Laboratories, Dubai, United Arab Emirates3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lode","family":"Wyns","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Ultrastructure, Vrije Universiteit Brussel, B-1640 St. Genesius Rode,1and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serge","family":"Muyldermans","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Ultrastructure, Vrije Universiteit Brussel, B-1640 St. Genesius Rode,1and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Burton\n              G.\n              Osborne\n              N. 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