{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T04:41:18Z","timestamp":1782362478689,"version":"3.54.5"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            From whole-cell DNA of a clinical isolate of the enterobacterial species\n            <jats:italic>Rahnella aquatilis<\/jats:italic>\n            , a \u03b2-lactamase gene was cloned that encoded a chromosomally encoded Ambler class A enzyme, RAHN-1. RAHN-1, with a pI of 7.2, shares 76, 73, and 71% amino acid identity with the extended-spectrum \u03b2-lactamase of chromosomal origin from\n            <jats:italic>Serratia fonticola<\/jats:italic>\n            and with the plasmid-mediated \u03b2-lactamases CTX-M-2 and CTX-M-1, respectively. The hydrolysis spectrum of the clavulanic acid-inhibited RAHN-1 was expanded to cephalosporins such as cefuroxime, cefotaxime, and ceftriaxone, but not to ceftazidime. Its expression was not inducible.\n          <\/jats:p>","DOI":"10.1128\/aac.45.10.2965-2968.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"2965-2968","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Biochemical-Genetic Characterization of the Chromosomally Encoded Extended-Spectrum Class A \u03b2-Lactamase from\n            <i>Rahnella aquatilis<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"Samuel","family":"Bellais","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Fortineau","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean W.","family":"Decousser","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.33.1.63"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(92)90121-S"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.2.509"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/301623a0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_8_2","first-page":"349","article-title":"Two epidemiologically related cases of Rahnella aquatilis bacteremia.","volume":"17","author":"Caroff N.","year":"1998","unstructured":"Caroff N. Chamoux C. Le Gallou F. Espaze E. Gavini F. Gautreau D. Richet H. Reynaud A. Two epidemiologically related cases of Rahnella aquatilis bacteremia. Eur. J. Clin. Microbiol. Infect. Dis. 17 1998 349 352","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/37.5.931"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a021399"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/3.6.379"},{"key":"e_1_3_2_12_2","first-page":"163","article-title":"Rahnella aquatilis, nouveau membre de la famille des Enterobacteriaceae.","volume":"130","author":"Izard D.","year":"1979","unstructured":"Izard D. Gavini F. Trinel P. A. Leclerc H. Rahnella aquatilis, nouveau membre de la famille des Enterobacteriaceae. Ann. Microbiol. (Paris) 130A 1979 163 177","journal-title":"Ann. Microbiol. (Paris)"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.1995.1.285"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.11.2294"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.11.2706-2708.1994"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3300581"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.19.1.185"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02505093"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.2.307"},{"key":"e_1_3_2_20_2","unstructured":"National Committee for Clinical Laboratory Standards\n          Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically\n          5th ed. Approved standard M7\u2013A4\n          2000\n          National Committee for Clinical Laboratory Standards\n          Wayne Pa"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(97)00020-4"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(94)90048-5"},{"key":"e_1_3_2_23_2","first-page":"79","article-title":"Cloning and nucleotide sequencing of the gene encoding the \u03b2-lactamase from Citrobacter diversus.","volume":"67","author":"Perilli M.","year":"1991","unstructured":"Perilli M. Franceschini N. Segatore B. Amicosante G. Oratore A. Duez C. Joris B. Fr\u00e8re J.-M. Cloning and nucleotide sequencing of the gene encoding the \u03b2-lactamase from Citrobacter diversus. FEMS Microbiol. Lett. 67 1991 79 84","journal-title":"FEMS Microbiol. 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