{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:34:50Z","timestamp":1791293690351,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Susceptibilities to 13 antimicrobial agents were determined by measurement of MICs for 60 isolates of\n            <jats:italic>Streptococcus bovis<\/jats:italic>\n            from blood cultures. Thirty-eight isolates (63.3%) had high-level resistance to erythromycin (MICs, \u2265128 \u03bcg\/ml). Among the 38 erythromycin-resistant strains, 21 isolates (55%) had inducible resistance to macrolides-lincosamides-streptogramin B (iMLS isolates) and 17 (45%) had constitutive resistance to macrolides-lincosamides-streptogramin B (cMLS isolates). Tetracycline resistance was also found among all of the erythromycin-resistant strains. None of the strains displayed resistance to penicillin, chloramphenicol, or vancomycin. Detection of erythromycin resistance genes by PCR and sequencing indicated that all 17 cMLS isolates were positive for the\n            <jats:italic>ermB<\/jats:italic>\n            gene and that 7 of 21 iMLS isolates carried the\n            <jats:italic>ermB<\/jats:italic>\n            gene and the remaining 14 iMLS isolates carried the\n            <jats:italic>ermT<\/jats:italic>\n            gene. Sequence analysis of amplified partial\n            <jats:italic>ermB<\/jats:italic>\n            fragments (594 bp) from\n            <jats:italic>S. bovis<\/jats:italic>\n            isolates revealed a 99.8% nucleotide identity and a 100% amino acid homology compared with the sequences from gene banks. The sequences of amplified fragments with primers targeted for\n            <jats:italic>ermC<\/jats:italic>\n            were shown to be very similar to that of\n            <jats:italic>ermGT<\/jats:italic>\n            (\n            <jats:italic>ermT<\/jats:italic>\n            ) from\n            <jats:italic>Lactobacillus reuteri<\/jats:italic>\n            (98.5% nucleotide identity). This is the first report to describe the detection of the\n            <jats:italic>ermT<\/jats:italic>\n            class of erythromycin resistance determinants in\n            <jats:italic>S. bovis<\/jats:italic>\n            . The high rate of inducible erythromycin resistance among\n            <jats:italic>S. bovis<\/jats:italic>\n            isolates in Taiwan was not reported before. The iMLS\n            <jats:italic>S. bovis<\/jats:italic>\n            isolates were shown to be heterogeneous by randomly amplified polymorphic DNA analysis. These results indicate that the prevalence of inducible erythromycin resistance in\n            <jats:italic>S. bovis<\/jats:italic>\n            in Taiwan is very high and that most of the resistant strains carry the\n            <jats:italic>ermT<\/jats:italic>\n            or the\n            <jats:italic>ermB<\/jats:italic>\n            gene.\n          <\/jats:p>","DOI":"10.1128\/aac.45.12.3362-3365.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"3362-3365","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["High Prevalence of Inducible Erythromycin Resistance among\n            <i>Streptococcus bovis<\/i>\n            Isolates in Taiwan"],"prefix":"10.1128","volume":"45","author":[{"given":"Lee-Jene","family":"Teng","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->School of Medical Technology, National Taiwan University College of Medicine,1 and"},{"name":"<!--label omitted: 2-->Department of Laboratory Medicine, National Taiwan University Hospital,2 Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po-Ren","family":"Hsueh","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Laboratory Medicine, National Taiwan University Hospital,2 Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen-Wu","family":"Ho","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->School of Medical Technology, National Taiwan University College of Medicine,1 and"},{"name":"<!--label omitted: 2-->Department of Laboratory Medicine, National Taiwan University Hospital,2 Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwen-Tay","family":"Luh","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Laboratory Medicine, National Taiwan 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