{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:09:55Z","timestamp":1791846595621,"version":"4.3.4"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Multidrug-resistant strain\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    BM4454 was isolated from a patient with a urinary tract infection. The\n                    <jats:italic>adeB<\/jats:italic>\n                    gene, which encodes a resistance-nodulation-cell division (RND) protein, was detected in this strain by PCR with two degenerate oligodeoxynucleotides. Insertional inactivation of\n                    <jats:italic>adeB<\/jats:italic>\n                    in BM4454, which generated BM4454-1, showed that the corresponding protein was responsible for aminoglycoside resistance and was involved in the level of susceptibility to other drugs including fluoroquinolones, tetracyclines, chloramphenicol, erythromycin, trimethoprim, and ethidium bromide. Study of ethidium bromide accumulation in BM4454 and BM4454-1, in the presence or in the absence of carbonyl cyanide\n                    <jats:italic>m<\/jats:italic>\n                    -chlorophenylhydrazone, demonstrated that AdeB was responsible for the decrease in intracellular ethidium bromide levels in a proton motive force-dependent manner. The\n                    <jats:italic>adeB<\/jats:italic>\n                    gene was part of a cluster that included\n                    <jats:italic>adeA<\/jats:italic>\n                    and\n                    <jats:italic>adeC<\/jats:italic>\n                    which encodes proteins homologous to membrane fusion and outer membrane proteins of RND-type three-component efflux systems, respectively. The products of two upstream open reading frames encoding a putative two-component regulatory system might be involved in the regulation of expression of the\n                    <jats:italic>adeABC<\/jats:italic>\n                    gene cluster.\n                  <\/jats:p>","DOI":"10.1128\/aac.45.12.3375-3380.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:06:42Z","timestamp":1027750002000},"page":"3375-3380","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":505,"title":["Resistance-Nodulation-Cell Division-Type Efflux Pump Involved in Aminoglycoside Resistance in\n                    <i>Acinetobacter baumannii<\/i>\n                    Strain BM4454"],"prefix":"10.1128","volume":"45","author":[{"given":"Sophie","family":"Magnet","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Unite\u0301 des Agents Antibacte\u0301riens, Institut Pasteur, 75724 Paris Cedex 15,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Courvalin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Unite\u0301 des Agents Antibacte\u0301riens, Institut Pasteur, 75724 Paris Cedex 15,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Lambert","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Unite\u0301 des Agents Antibacte\u0301riens, Institut Pasteur, 75724 Paris Cedex 15,1 and"},{"name":"<!--label omitted: 2-->Centre d'Etudes Pharmaceutiques, Cha\u0302tenay-Malabry,2 France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)46956-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.22.5836-5843.1998"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.9.2.148"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.22.2.323"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.7.2274-2280.1997"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.3.664-671.2000"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.5.1223-1228.2000"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(83)90066-5"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-141-3-611"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","unstructured":"Hood\n              J.\n              Amyes\n              S. 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