{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T07:16:29Z","timestamp":1793085389854,"version":"4.5.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2001,3,1]],"date-time":"2001-03-01T00:00:00Z","timestamp":983404800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>Mycobacterium bovis P55<\/jats:italic>\n            gene, located downstream from the gene that encodes the immunogenic lipoprotein P27, has been characterized. The gene was identical to the open reading frame of the Rv1410c gene in the genome of\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            H37Rv, annotated as a probable drug efflux protein. Genes similar to\n            <jats:italic>P55<\/jats:italic>\n            were present in all species of the\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            complex and other mycobacteria such as\n            <jats:italic>Mycobacterium leprae<\/jats:italic>\n            and\n            <jats:italic>Mycobacterium avium<\/jats:italic>\n            . By Western blotting, P55 was located in the membrane fraction of\n            <jats:italic>M. bovis<\/jats:italic>\n            . When transformed into\n            <jats:italic>Mycobacterium smegmatis<\/jats:italic>\n            after cloning, P55 conferred aminoglycoside and tetracycline resistance. The levels of resistance to streptomycin and tetracycline conferred by P55 were decreased in the presence of the protonophore carbonyl cyanide\n            <jats:italic>m<\/jats:italic>\n            -chlorophenylhydrazone and the pump inhibitors verapamil and reserpine.\n            <jats:italic>M. smegmatis<\/jats:italic>\n            cells expressing the plasmid-encoded P55 accumulated less tetracycline than the control cells. We conclude that P55 is a membrane protein implicated in aminoglycoside and tetracycline efflux in mycobacteria.\n          <\/jats:p>","DOI":"10.1128\/aac.45.3.800-804.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"800-804","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":115,"title":["Characterization of P55, a Multidrug Efflux Pump in\n            <i>Mycobacterium bovis<\/i>\n            and\n            <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"Pedro E. 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