{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:03:15Z","timestamp":1790866995010,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2001,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>mdt<\/jats:italic>\n            (A) gene, previously designated\n            <jats:italic>mef214<\/jats:italic>\n            , from\n            <jats:italic>Lactococcus lactis<\/jats:italic>\n            subsp.\n            <jats:italic>lactis<\/jats:italic>\n            plasmid pK214 encodes a protein [Mdt(A) (multiple drug transporter)] with 12 putative transmembrane segments (TMS) that contain typical motifs conserved among the efflux proteins of the major facilitator superfamily. However, it also has two C-motifs (conserved in the fifth TMS of the antiporters) and a putative ATP-binding site. Expression of the cloned\n            <jats:italic>mdt<\/jats:italic>\n            (A) gene decreased susceptibility to macrolides, lincosamides, streptogramins, and tetracyclines in\n            <jats:italic>L. lactis<\/jats:italic>\n            and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , but not in\n            <jats:italic>Enterococcus faecalis<\/jats:italic>\n            or in\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            . Glucose-dependent efflux of erythromycin and tetracycline was demonstrated in\n            <jats:italic>L. lactis<\/jats:italic>\n            and in\n            <jats:italic>E. coli<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.45.4.1109-1114.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"1109-1114","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Mdt(A), a New Efflux Protein Conferring Multiple Antibiotic Resistance in\n            <i>Lactococcus lactis<\/i>\n            and\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"Vincent","family":"Perreten","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Center for Adaptation Genetics and Drug Resistance1 and"},{"name":"<!--label omitted: 2-->Departments of Molecular Biology and Microbiology2"},{"name":"<!--label omitted: 3-->Laboratory of Food Microbiology, Institute of Food Science, Department of Agriculture and Food Science, Eidgeno\u0308ssische Technische Hochschule Zu\u0308rich, ETH-Zentrum, CH-8092 Zu\u0308rich, Switzerland3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska V.","family":"Schwarz","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Laboratory of Food Microbiology, Institute of Food Science, Department of Agriculture and Food Science, Eidgeno\u0308ssische Technische Hochschule Zu\u0308rich, ETH-Zentrum, CH-8092 Zu\u0308rich, Switzerland3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Teuber","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Laboratory of Food Microbiology, Institute of Food Science, Department of Agriculture and Food Science, Eidgeno\u0308ssische Technische Hochschule Zu\u0308rich, ETH-Zentrum, CH-8092 Zu\u0308rich, Switzerland3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart B.","family":"Levy","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Adaptation Genetics and Drug Resistance1 and"},{"name":"<!--label omitted: 2-->Departments of Molecular Biology and Microbiology2"},{"name":"<!--label omitted: 4-->and of Medicine,4 Tufts University School of Medicine, Boston, Massachusetts 02111, and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.22.5836-5843.1998"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.46.3.549-552.1983"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.22.6957-6964.1994"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.44.26092"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1006\/jmbi.1993.1126","article-title":"Gene organization, primary structure and RNA processing analysis of a ribosomal RNA operon in Lactococcus lactis.","volume":"230","author":"Chiaruttini C.","year":"1993","unstructured":"Chiaruttini C. 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