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These proteins were hardly detected at concentrations that saturate all PBPs in clinical isolates and showed, after densitometric analysis, 50-fold-lower radiotracer binding. Resistance was related to mosaic structure in all HMM PBP-coding genes, where critical region replacement was complemented not only by substitutions already reported for the closely related\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            but also by other specific replacements that are presumably close to the active-site serine. Mosaic structure was also presumed in a\n            <jats:italic>pbp1a<\/jats:italic>\n            -sensitive strain used for comparison, confirming that these structures do not unambiguously imply, by themselves, detectable critical changes in the kinetic properties of these proteins.\n          <\/jats:p>","DOI":"10.1128\/aac.45.7.2075-2081.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"2075-2081","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["All Detectable High-Molecular-Mass Penicillin-Binding Proteins Are Modified in a High-Level \u03b2-Lactam-Resistant Clinical Isolate of\n            <i>Streptococcus mitis<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"Ana","family":"Amoroso","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de resistencia microbiana, Ca\u0301tedra de Microbiolog\u0131\u0301a. Departamento de Microbiolog\u0131\u0301a, Inmunolog\u0131\u0301a y Biotecnolog\u0131\u0301a, Facultad de Farmacia y Bioqu\u0131\u0301mica, Universidad de Buenos Aires, Buenos Aires, Argentina,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Demares","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de resistencia microbiana, Ca\u0301tedra de Microbiolog\u0131\u0301a. Departamento de Microbiolog\u0131\u0301a, Inmunolog\u0131\u0301a y Biotecnolog\u0131\u0301a, Facultad de Farmacia y Bioqu\u0131\u0301mica, Universidad de Buenos Aires, Buenos Aires, Argentina,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Mollerach","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de resistencia microbiana, Ca\u0301tedra de Microbiolog\u0131\u0301a. Departamento de Microbiolog\u0131\u0301a, Inmunolog\u0131\u0301a y Biotecnolog\u0131\u0301a, Facultad de Farmacia y Bioqu\u0131\u0301mica, Universidad de Buenos Aires, Buenos Aires, Argentina,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Gutkind","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratorio de resistencia microbiana, Ca\u0301tedra de Microbiolog\u0131\u0301a. 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