{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T00:44:33Z","timestamp":1793321073515,"version":"4.5.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2001,8,1]],"date-time":"2001-08-01T00:00:00Z","timestamp":996624000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Ochrobactrum anthropi<\/jats:italic>\n            , formerly known as CDC group Vd, is an oxidase-producing, gram-negative, obligately aerobic, non-lactose-fermenting bacillus of low virulence that occasionally causes human infections. It is highly resistant to all \u03b2-lactams except imipenem. A clinical isolate, SLO74, and six reference strains were tested. MICs of penicillins, aztreonam, and most cephalosporins tested, including cefotaxime and ceftazidime, were &gt;128 \u03bcg\/ml and of cefepime were 64 to &gt;128 \u03bcg\/ml. Clavulanic acid was ineffective and tazobactam had a weak effect in association with piperacillin. Two genes,\n            <jats:italic>ampR<\/jats:italic>\n            and\n            <jats:italic>ampC<\/jats:italic>\n            , were cloned by inserting restriction fragments of genomic DNA from the clinical strain\n            <jats:italic>O. anthropi<\/jats:italic>\n            SLO74 into pBK-CMV to give the recombinant plasmid pBK-OA1. The pattern of resistance to \u03b2-lactams of this clone was similar to that of the parental strain, except for its resistance to cefepime (MIC, 0.5 \u03bcg\/ml). The deduced amino acid sequence of the AmpC \u03b2-lactamase (pI, 8.9) was only 41 to 52% identical to the sequence of other chromosomally encoded and plasmid-encoded class C \u03b2-lactamases. The kinetic properties of this \u03b2-lactamase were typical for this class of \u03b2-lactamases. Upstream from the\n            <jats:italic>ampC<\/jats:italic>\n            gene, the\n            <jats:italic>ampR<\/jats:italic>\n            gene encodes a protein with a sequence that is 46 to 62% identical to those of other AmpR proteins and with an amino-terminal DNA-binding domain typical of transcriptional activators of the Lys-R family. The deduced amino acid sequences of the\n            <jats:italic>ampC<\/jats:italic>\n            genes of the six reference strains were 96 to 99% identical to the sequence of the clinical strain. The \u03b2-lactamase characterized from strain SLO74 was named OCH-1 (gene,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OCH-I<\/jats:sub>\n            ).\n          <\/jats:p>","DOI":"10.1128\/aac.45.8.2324-2330.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"2324-2330","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Molecular Characterization of Chromosomal Class C \u03b2-Lactamase and Its Regulatory Gene in\n            <i>Ochrobactrum anthropi<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"David","family":"Nadjar","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie, Ho\u0302pital Tenon, UFR Saint-Antoine,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Labia","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->CNRS-UBO-MHN, Unite\u0301 FRE 2125, Quimper,2 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Cerceau","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->CNRS-UBO-MHN, Unite\u0301 FRE 2125, Quimper,2 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Bizet","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Collection de l'Institut Pasteur, Institut Pasteur,3 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Philippon","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Service de Bacte\u0301riologie, CHU Cochin,4 Paris, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Arlet","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie, Ho\u0302pital Tenon, UFR Saint-Antoine,1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.13.4.695"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.2.153"},{"key":"e_1_3_2_4_2","first-page":"258","article-title":"Sensibilit\u00e9 compar\u00e9e de Ochrobactrum anthropi, Agrobacterium tumefaciens, Alcaligenes faecalis, Alcaligenes denitrificans subsp. denitrificans, Alcaligenes denitrificans subsp. xylosoxydans et Bordetella bronchiseptica vis-\u00e0-vis de 35 antibiotiques dont 17 \u03b2-lactamamines.","volume":"43","author":"Bizet C.","year":"1995","unstructured":"Bizet C. Bizet J. Sensibilit\u00e9 compar\u00e9e de Ochrobactrum anthropi, Agrobacterium tumefaciens, Alcaligenes faecalis, Alcaligenes denitrificans subsp. denitrificans, Alcaligenes denitrificans subsp. xylosoxydans et Bordetella bronchiseptica vis-\u00e0-vis de 35 antibiotiques dont 17 \u03b2-lactamamines. Path. Biol. 43 1995 258 263","journal-title":"Path. Biol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1093\/clinids\/17.3.516","article-title":"Necrotizing fasciitis, bacteremia, and multiorgan failure caused by Ochrobactrum anthropi.","volume":"17","author":"Brivet F.","year":"1993","unstructured":"Brivet F. Guibert M. Kiredjian M. Dormont J. Necrotizing fasciitis, bacteremia, and multiorgan failure caused by Ochrobactrum anthropi. Clin. Infect. Dis. 17 1993 516 518","journal-title":"Clin. Infect. 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