{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:41:19Z","timestamp":1790883679917,"version":"4.1.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2001,9,1]],"date-time":"2001-09-01T00:00:00Z","timestamp":999302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A total of 113 blood culture isolates of\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            from 10 hospitals in northern Taiwan were studied for SHV and TEM \u03b2-lactamase production.\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV<\/jats:sub>\n            was amplified from all isolates by PCR. TEM-type resistance, was found in 32 of the isolates and was of the TEM-1 type in all isolates. SHV-1, -2, -5, -11, and -12 and two novel enzymes were identified. These novel enzymes were designated SHV-25 and SHV-26 and had pIs of 7.5 and 7.6, respectively. Amino acid differences in comparison to the amino acid sequence of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-1<\/jats:sub>\n            were found at positions T18A (ThrACC\u2192AlaGCC), L35Q (LeuCTA\u2192GluCAA), and M129V (MetATG\u2192ValGTG) for SHV-25 and at position A187T (AlaGCC\u2192ThrACC) for SHV-26. The results of substrate profiles and MIC determinations showed that the novel enzymes did not hydrolyze extended-spectrum cephalosporins, rendering the isolates susceptible to these agents. Inhibition profiles revealed that the 50% inhibitory concentration for SHV-26 was higher than those for SHV-1 and SHV-25, resulting in an intermediate resistance to amoxicillin-clavulanic acid. Forty-nine ribotypes were identified, suggesting that major clonal spread had not occurred in any of the hospitals. According to the amino acid sequence, SHV \u03b2-lactamases in Taiwan may basically be derived through stepwise mutation from SHV-1 or SHV-11 and further subdivided by four routes. The stepwise mutations initiated from SHV-1 or SHV-11 to SHV-2, SHV-5, and SHV-12 comprise the evolutionary change responsible for extended-spectrum \u03b2-lactamase (ESBL) production in Taiwan. The stepwise mutations that lead to a non-ESBL (SHV-25) and the \u03b2-lactamase (SHV-26) with reduced susceptibility to clavulanic acid are possibly derived from SHV-11 and SHV-1, respectively. The results suggest a stepwise evolution of SHV \u03b2-lactamases in Taiwan.\n          <\/jats:p>","DOI":"10.1128\/aac.45.9.2407-2413.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"2407-2413","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Diversity of SHV and TEM \u03b2-Lactamases in\n            <i>Klebsiella pneumoniae<\/i>\n            : Gene Evolution in Northern Taiwan and Two Novel \u03b2-Lactamases, SHV-25 and SHV-26"],"prefix":"10.1128","volume":"45","author":[{"given":"Feng-Yee","family":"Chang","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Division of Infectious Disease and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. K.","family":"Siu","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Division of Clinical Research, National Health Research Institute,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Phone","family":"Fung","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Section of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, and National Yang-Ming University,3 Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Hua","family":"Huang","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Infectious Disease and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monto","family":"Ho","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Division of Clinical Research, National Health Research Institute,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.8.2230-2230.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1179\/joc.1999.11.2.97","article-title":"Extended-spectrum beta-lactamase-producing strains of Enterobacter cloacae transferring resistance to cefotaxime and ceftazidime.","volume":"11","author":"Blahova J.","year":"1999","unstructured":"Blahova J. Kralikova K. Krcmery V. Sr. Schafer V. Extended-spectrum beta-lactamase-producing strains of Enterobacter cloacae transferring resistance to cefotaxime and ceftazidime. J. Chemother. 11 1999 97 102","journal-title":"J. Chemother."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.10.3636-3645.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.1.6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.12.2593"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.557"},{"key":"e_1_3_2_8_2","unstructured":"Mabilat\n              C.\n              Goussard\n              S.\n          PCR detection and identification of genes for extended-spectrum \u03b2-lactamases\n          Diagnostic molecular microbiology: principles and applications.\n              Persing\n              D. H.\n              Smith\n              T. F.\n              Tenover\n              F. C.\n              White\n              T. J.\n          1993\n          553\n          563\n          American Society for Microbiology\n          Washington D.C."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.8.2069"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1771-1777.2000"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.7.1669"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1099\/00221287-94-1-55","article-title":"Identification of beta-lactamases by analytical isoelectric focusing: correlation with bacterial taxonomy.","volume":"94","author":"Matthew M.","year":"1976","unstructured":"Matthew M. Harris A. M. Identification of beta-lactamases by analytical isoelectric focusing: correlation with bacterial taxonomy. J. Gen. Microbiol. 94 1976 55 67","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_13_2","unstructured":"National Committee for Clinical Laboratory Standards\n          Performance standards for antimicrobial disk susceptibility tests. Approved standard M2\u2013A6.\n          1997\n          National Committee for Clinical Laboratory Standards\n          Wayne Pa"},{"key":"e_1_3_2_14_2","unstructured":"National Committee for Clinical Laboratory Standards\n          Performance standards for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7\u2013A4.\n          1997\n          National Committee for Clinical Laboratory Standards\n          Wayne Pa"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.3.647"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1089\/mdr.1999.5.189","article-title":"Extended-spectrum beta-lactamases in clinical isolates of enterobacteria in Mexico.","volume":"5","author":"Silva J.","year":"1999","unstructured":"Silva J. Aguilar C. Becerra Z. Lopez-Antunano F. Garcia R. Extended-spectrum beta-lactamases in clinical isolates of enterobacteria in Mexico. Microb. Drug Resist. 5 1999 189 193","journal-title":"Microb. Drug Resist."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4020-4027.1999"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1007\/s150100050041","article-title":"Occurrence of extended-spectrum beta-lactamases (ESBL) in Dutch hospitals.","volume":"27","author":"Stobberingh E. E.","year":"1999","unstructured":"Stobberingh E. E. Arends J. Hoogkamp-Korstanje J. A. Goessens W. H. Visser M. R. Buiting A. G. Debets-Ossenkopp Y. J. van Ketel R. J. van Ogtrop M. L. Sabbe L. J. Voorn G. P. Winter H. L. van Zeijl J. H. Occurrence of extended-spectrum beta-lactamases (ESBL) in Dutch hospitals. Infection 27 1999 348 354","journal-title":"Infection"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.45.9.2407-2413.2001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.45.9.2407-2413.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T17:12:05Z","timestamp":1645463525000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.45.9.2407-2413.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,9]]},"references-count":18,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2001,9]]}},"alternative-id":["10.1128\/AAC.45.9.2407-2413.2001"],"URL":"https:\/\/doi.org\/10.1128\/aac.45.9.2407-2413.2001","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,9]]},"assertion":[{"value":"2000-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-05-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}