{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:33:18Z","timestamp":1761154398461},"reference-count":47,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Biofilm formation mediated by polysaccharide intercellular adhesin (PIA) is the major virulence factor of\n            <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n            and is often associated with methicillin resistance. Transposon Tn\n            <jats:italic>917<\/jats:italic>\n            insertions leading to a biofilm-negative phenotype in the biofilm-producing\n            <jats:italic>S. epidermidis<\/jats:italic>\n            strain 1457 (\n            <jats:italic>mecA<\/jats:italic>\n            -negative) were transferred into the methicillin-resistant, biofilm-producing\n            <jats:italic>S. epidermidis<\/jats:italic>\n            1057 (\n            <jats:italic>mecA<\/jats:italic>\n            -positive) by transduction. According to their phenotypes and genotypes, the mutants could be separated into genetic classes I to IV (D. Mack, H. Rohde, S. Dobinsky, J. Riedewald, M. Nedelmann, J. K. M. Knobloch, H.-A. Elsner, and H. H. Feucht, Infect. Immun. 68:3799\u20133807, 2000). All transductants of\n            <jats:italic>S. epidermidis<\/jats:italic>\n            1057 had phenotypes for biofilm formation similar to those of the corresponding mutants of\n            <jats:italic>S. epidermidis<\/jats:italic>\n            1457. With a\n            <jats:italic>mecA<\/jats:italic>\n            -specific probe, identical hybridization patterns were observed for wild-type\n            <jats:italic>S. epidermidis<\/jats:italic>\n            1057 and all the transductants. There were minor changes in oxacillin MICs for Class II and III transductants compared to those for wild-type\n            <jats:italic>S. epidermidis<\/jats:italic>\n            1057. On population analysis,\n            <jats:italic>S. epidermidis<\/jats:italic>\n            1057 displayed a heterogeneous expression type of resistance with an oxacillin MIC of \u22656 \u03bcg\/ml for more than 90% of the cells. An almost identical profile was observed with biofilm-negative class I mutants, where the transposon insertions inactivate the\n            <jats:italic>icaADBC<\/jats:italic>\n            gene locus essential for PIA synthesis. In contrast, class III mutants were more sensitive to oxacillin with a MIC of \u22641 \u03bcg\/ml for more than 90% of the cells. The class IV mutant displayed homogenous resistance with a MIC of \u226550 \u03bcg\/ml for more than 90% of the cells. On oxacillin gradient plates, the class II mutant displayed decreased resistance. Apparently, different independent mutations leading to a biofilm-negative phenotype of\n            <jats:italic>S. epidermidis<\/jats:italic>\n            by influencing expression of\n            <jats:italic>icaADBC<\/jats:italic>\n            on the level of transcription significantly influence the expression of methicillin resistance. However, transcription of\n            <jats:italic>mecA<\/jats:italic>\n            was not significantly altered in the different transductants compared to the wild type, independent of\n            <jats:italic>mecA<\/jats:italic>\n            induction with oxacillin, indicating that other mechanisms influencing phenotypic expression of methicillin resistance are involved.\n          <\/jats:p>","DOI":"10.1128\/aac.46.1.178-183.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:06:42Z","timestamp":1027764402000},"page":"178-183","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Differential Expression of Methicillin Resistance by Different Biofilm-Negative\n            <i>Staphylococcus epidermidis<\/i>\n            Transposon Mutant Classes"],"prefix":"10.1128","volume":"46","author":[{"given":"Dietrich","family":"Mack","sequence":"first","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, 20246 Hamburg, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Axel","family":"Sabottke","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, 20246 Hamburg, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Dobinsky","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, 20246 Hamburg, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holger","family":"Rohde","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, 20246 Hamburg, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias A.","family":"Horstkotte","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, 20246 Hamburg, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes K.-M.","family":"Knobloch","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Immunologie, Universita\u0308tsklinikum Hamburg-Eppendorf, 20246 Hamburg, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.10.2231"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050023"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.36.7.1367"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.781"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.6.1153"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.37.1.318-326.1982"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.22.6.996-1006.1985"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/33.1.7"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.4.632"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.11.2590"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.1999.5.163"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.6.1616-1623.2000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)36594-1"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/314762"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.877-880.2000"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.20.6294-6301.1997"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.29.18586"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02548.x"},{"key":"e_1_3_2_20_2","first-page":"1526","year":"1999","unstructured":"Jorgensen, J. H., J. D. Turnidge, and J. A. Washington. 1999. Antibacterial susceptibility tests: dilution and disk diffusion methods, p.1526\u20131543. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C.","journal-title":"Manual of clinical microbiology, 7th ed."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.8.2624-2633.2001"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.11.2355"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.3.815-824.2001"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-6701(99)90074-9"},{"key":"e_1_3_2_25_2","first-page":"307","year":"2000","unstructured":"Mack, D., K. Bartscht, S. Dobinsky, M. A. Horstkotte, K. Kiel, J. K. M. Knobloch, and P. Sch\u00e4fer. 2000. Staphylococcal factors involved in adhesion and biofilm formation on biomaterials, p.307\u2013330. In Y. H. An and R. J. Friedman (ed.), Handbook for studying bacterial adhesion: principles, methods, and applications. Humana Press, Totowa, N.J.","journal-title":"Handbook for studying bacterial adhesion: principles, methods, and applications"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)36592-8"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.1.175-183.1996"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.4.881"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.8.3244-3253.1994"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.2.1004-1008.1999"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.3799-3807.2000"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.5.2048-2057.1992"},{"key":"e_1_3_2_33_2","first-page":"411","volume":"26","year":"1994","unstructured":"Mack, D., N. Siemssen, and R. Laufs. 1994. Identification of a cell cluster associated antigen specific for plastic-adherent Staphylococcus epidermidis which is functionally related to intercellular adhesion. Zentbl. Bakteriol. Suppl.26:411\u2013413.","journal-title":"Zentbl. Bakteriol. Suppl."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.22.6944-6948.1997"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.6.1251"},{"key":"e_1_3_2_36_2","first-page":"109","volume":"184","year":"1995","unstructured":"Mempel, M., E. M\u00fcller, R. Hoffmann, H. Feucht, R. Laufs, and L. Gr\u00fcter. 1995. Variable degree of slime production is linked to different levels of beta-lactam susceptibility in Staphylococcus epidermidis phase variants. Med. Microbiol. Immunol. (Berlin)184:109\u2013113.","journal-title":"Med. Microbiol. Immunol. (Berlin)"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0934-8840(98)80151-5"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/19.2.231"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.5.2627-2632.1999"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.5.2656-2659.1999"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.4.724"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-46-9-773"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.1.124"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1053\/ic.1999.v27.a98878"},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0924-8579(96)00346-9","volume":"8","year":"1997","unstructured":"Vasilieva, E., H. Labischinski, and B. Berger-B\u00e4chi. 1997. Identification and mapping of new chromosomal sites affecting response to \u03b2-lactams in Staphylococcus aureus. Int. J. Antimicrob. Agents8:13\u201321.","journal-title":"Int. J. Antimicrob. Agents"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.20.6036-6042.1996"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.3.890-896.1997"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01353.x"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.46.1.178-183.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.46.1.178-183.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T17:27:31Z","timestamp":1645464451000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.46.1.178-183.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1128\/AAC.46.1.178-183.2002"],"URL":"https:\/\/doi.org\/10.1128\/aac.46.1.178-183.2002","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1]]},"assertion":[{"value":"2001-05-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-10-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}