{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T01:14:11Z","timestamp":1790990051686,"version":"4.1.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Desulfovibrio<\/jats:italic>\n            spp. are gram-negative anaerobes phylogenetically related to\n            <jats:italic>Bacteroides<\/jats:italic>\n            spp., which are rarely isolated and which are mostly isolated from intra-abdominal abscesses.\n            <jats:italic>Desulfovibrio desulfuricans<\/jats:italic>\n            clinical isolate D3 had a clavulanic acid-inhibited \u03b2-lactam resistance profile and was resistant to some expanded-spectrum cephalosporins. A \u03b2-lactamase gene,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>DES-1<\/jats:sub>\n            , was cloned from whole-cell DNA of isolate D3 and expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . Purified \u03b2-lactamase DES-1, with a pI value of 9.1, had a relative molecular mass of ca. 31 kDa and a mature protein of 288 amino acids. DES-1 was distantly related to Ambler class A \u03b2-lactamases and most closely related to PenA from\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            (48% amino acid identity). It was weakly related to class A \u03b2-lactamases CblA, CepA, CfxA, and CfxA2 from other anaerobic species,\n            <jats:italic>Bacteroides<\/jats:italic>\n            spp. and\n            <jats:italic>Prevotella intermedia<\/jats:italic>\n            . Its hydrolysis spectrum included amino- and ureidopenicillins, narrow-spectrum cephalosporins, ceftriaxone, and cefoperazone.\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>DES-1<\/jats:sub>\n            -like genes were not identified in phylogenetically related\n            <jats:italic>Desulfovibrio fairfieldensis<\/jats:italic>\n            isolates. However, they were found in some but not all\n            <jats:italic>D. desulfuricans<\/jats:italic>\n            strains, thus suggesting that these genes may be present in a given\n            <jats:italic>D. desulfuricans<\/jats:italic>\n            subspecies.\n          <\/jats:p>","DOI":"10.1128\/aac.46.10.3215-3222.2002","type":"journal-article","created":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T19:23:02Z","timestamp":1032204182000},"page":"3215-3222","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Biochemical-Genetic Analysis and Distribution of DES-1, an Ambler Class A Extended-Spectrum \u03b2-Lactamase from\n            <i>Desulfovibrio desulfuricans<\/i>"],"prefix":"10.1128","volume":"46","author":[{"given":"Anne-Sophie","family":"Morin","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francine","family":"Mory","sequence":"additional","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie, Ho\u0302pital Central, 54035 Nancy Cedex"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Labia","sequence":"additional","affiliation":[{"name":"UMR175 CNRS Chimie et Biologie des Substances Actives, 29000 Quimper, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"273","volume":"5","year":"1988","unstructured":"Aldridge, K. E., A. Henderberg, D. D. Schiro, and C. V. Sanders. 1988. Susceptibility of Bacteroides fragilis group isolates to broad spectrum \u03b2-lactams, clindamycin and metronidazole: rates of resistance, cross-resistance, and importance of \u03b2-lactamase production. Adv. Ther.5:273-282.","journal-title":"Adv. Ther."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.8.1546"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.1924-1930.1997"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1093\/clinids\/14.1.227","volume":"14","year":"1992","unstructured":"Baron, E. J., R. Bennion, J. Thompson, C. Strong, P. Summanen, M. McTeague, and S. M. Finegold. 1992. A microbiological comparison between acute and complicated appendicitis. Clin. Infect. Dis.14:227-231.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.2.546-552.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.7.3609-3619.1990"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"S88","DOI":"10.1086\/516237","volume":"25","year":"1997","unstructured":"Finegold, S. M., and H. Jousimies-Somer. 1997. Recently described clinically important anaerobic bacteria: medical aspects. Clin. Infect. Dis.25(Suppl. 2):S88-S93.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3144-3149.2000"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1990.tb01575.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/j.1365-2672.1988.tb01498.x","volume":"65","year":"1988","unstructured":"Gibson, G. R., G. T. MacFarlane, and J. H. Cummings. 1988. Occurrence of sulphate-reducing bacteria in human faeces and the relationship of dissimilatory sulphate reduction to methanogenesis in the large gut. J. Appl. Bacteriol.65:103-111.","journal-title":"J. Appl. Bacteriol."},{"key":"e_1_3_2_14_2","first-page":"175","volume":"16","year":"1993","unstructured":"Hentges, D. J. 1993. The anaerobic microflora of the human body. Clin. Infect. Dis.16(Suppl. 4):175-180.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43377-7"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.9.1725"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2432"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.10.2269"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01967803"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/9.6.1150"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.11.2294"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.145.3.1365-1373.1981"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.9.3076-3077.1999"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.7.1644"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/20.3.313"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.931-934.2000"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.10.2933-2935.2001"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050357"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00286188"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.8.2386-2389.2001"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3300581"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.2.307"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.7.1805-1808.1997"},{"key":"e_1_3_2_34_2","unstructured":"National Committee for Clinical Laboratory Standards. 2000. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically 5th ed. Approved standard M7-A4. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.5.962"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.10.4345-4350.1996"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.5.1028"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.8.2287-2298.2001"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.18.5270-5275.1995"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.3.622-632.2000"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.3.573"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.5.4.444-447.1977"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/24.Supplement_1.S110"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.11.2391"},{"key":"e_1_3_2_45_2","unstructured":"Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y."},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.8.1711"},{"key":"e_1_3_2_47_2","unstructured":"Swofford D. L. 1989. PAUP (version 3.0): phylogenetic analysis using parsimony. Illinois Natural History Survey Champaign."},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00199a049"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.7.1760-1764.1996"},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","unstructured":"Widdel F. and F. Bak. 1992. Gram negative mesophilic sulfate reducing bacteria p. 3352-3378. In A. Balows H. G. Tr\u00fcper M. Dworkin W. Harder and K. H. Schliefer (ed.) The prokaryotes vol. IV 2nd ed. Springer-Verlag New York N.Y.","DOI":"10.1007\/978-1-4757-2191-1_21"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1294"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.46.10.3215-3222.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.46.10.3215-3222.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T17:22:28Z","timestamp":1645464148000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.46.10.3215-3222.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,10]]},"references-count":50,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2002,10]]}},"alternative-id":["10.1128\/AAC.46.10.3215-3222.2002"],"URL":"https:\/\/doi.org\/10.1128\/aac.46.10.3215-3222.2002","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,10]]},"assertion":[{"value":"2001-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-07-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}