{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T12:20:44Z","timestamp":1780143644936,"version":"3.54.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Empedobacter brevis<\/jats:italic>\n            (formerly designated\n            <jats:italic>Flavobacterium breve<\/jats:italic>\n            ) is a gram-negative aerobe involved in nosocomial infections. The Ambler class B \u03b2-lactamase gene\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>EBR-1<\/jats:sub>\n            was cloned and expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            from\n            <jats:italic>E. brevis<\/jats:italic>\n            clinical strain ASS-1, which had reduced susceptibility to expanded-spectrum cephalosporins and carbapenems. Purified \u03b2-lactamase EBR-1 hydrolyzed penicillins, cephalosporins, and carbapenems efficiently but not aztreonam. Kinetic parameters of EBR-1 were similar to those of class B enzymes such as BlaB, IND-2, and GOB-1 identified from other\n            <jats:italic>Flavobacteriaceae<\/jats:italic>\n            species, except for meropenem, which was more hydrolyzed by \u03b2-lactamase GOB-1. EBR-1, with a pI of 8.0 and a relative molecular mass of ca. 25 kDa, was classified in functional subgroup 3a, which includes most of the class B \u03b2-lactamases. EBR-1, which belongs to molecular subclass B1 of metalloenzymes, shares 58, 57, and 42% amino acid identity with the most closely related \u03b2-lactamases, IND-1\/IND-2 from\n            <jats:italic>Chryseobacterium indologenes<\/jats:italic>\n            , CGB-1 from\n            <jats:italic>Chryseobacterium gleum<\/jats:italic>\n            , and BlaB from\n            <jats:italic>Chryseobacterium meningosepticum<\/jats:italic>\n            , respectively.\n          <\/jats:p>","DOI":"10.1128\/aac.46.10.3223-3227.2002","type":"journal-article","created":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T19:23:02Z","timestamp":1032204182000},"page":"3223-3227","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["EBR-1, a Novel Ambler Subclass B1 \u03b2-Lactamase from\n            <i>Empedobacter brevis<\/i>"],"prefix":"10.1128","volume":"46","author":[{"given":"Samuel","family":"Bellais","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Girlich","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Karim","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre Ce\u0301dex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Bellais S. 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