{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T18:22:44Z","timestamp":1793298164712,"version":"4.5.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Alterations within the penicillin-binding domain of penicillin-binding protein (PBP) genes\n            <jats:italic>pbp1a<\/jats:italic>\n            ,\n            <jats:italic>pbp2b<\/jats:italic>\n            , and\n            <jats:italic>pbp2x<\/jats:italic>\n            were determined for 15 Canadian isolates of\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            . All penicillin-nonsusceptible\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            isolates showed a variety of PBP 2X substitutions and contained a Thr445-Ala change after the PBP 2B SSN motif. Only isolates for which penicillin MICs were \u22650.5 \u03bcg\/ml had PBP 1A alterations near the STMK and SRN motifs. Sequence analysis revealed identical PBP 1A, PBP 2B, and PBP 2X substitution patterns among all isolates for which penicillin MICs were \u22651 \u03bcg\/ml.\n          <\/jats:p>","DOI":"10.1128\/aac.46.10.3261-3264.2002","type":"journal-article","created":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T19:23:02Z","timestamp":1032204182000},"page":"3261-3264","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Penicillin-Binding Protein 1A, 2B, and 2X Alterations in Canadian Isolates of Penicillin-Resistant\n            <i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1128","volume":"46","author":[{"given":"Kimberly A.","family":"Nichol","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine"},{"name":"Clinical Microbiology, Health Sciences Centre, Winnipeg, Manitoba R3A 1R9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George G.","family":"Zhanel","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine"},{"name":"Faculty of Pharmacy, University of Manitoba"},{"name":"Departments of Medicine"},{"name":"Clinical Microbiology, Health Sciences Centre, Winnipeg, Manitoba R3A 1R9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daryl J.","family":"Hoban","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine"},{"name":"Clinical Microbiology, Health Sciences Centre, Winnipeg, Manitoba R3A 1R9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1252"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.9.2267"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(99)00104-7"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1995.tb07433.x"},{"key":"e_1_3_2_6_2","first-page":"21","volume":"26","year":"1990","unstructured":"Chalkley, L. J., and H. J. Koornhof. 1990. Intra- and interspecific transformation of Streptococcus pneumoniae to penicillin resistance. J. Antimicrob. Chemother.26:21-28.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.22.8842"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00108.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"7518","DOI":"10.1093\/nar\/17.18.7518","volume":"17","year":"1989","unstructured":"Dowson, C. G., A. Hutchison, and B. G. Spratt. 1989. Nucleotide sequence of the penicillin-binding protein 2B gene of Streptococcus pneumoniae strain R6. Nucleic Acids Res.17:7518.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.45.100191.000345"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.4.829"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.4.553"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3107125"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1993.tb05954.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1111\/j.1365-2958.1989.tb00115.x","volume":"3","year":"1989","unstructured":"Laible, G., R. Hakenbeck, M. A. Sicard, B. Joris, and J. M. Ghuysen. 1989. Nucleotide sequences of the pbpX genes encoding the penicillin-binding proteins 2x from Streptococcus pneumoniae R6 and a cefotaxime-resistant mutant, C506. Mol. Microbiol.3:1337-1348.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb00821.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.13.4517-4523.1992"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05475.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.23.13403"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.4.859"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.6.1329"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.9.1938"},{"key":"e_1_3_2_23_2","unstructured":"Yao J. D. C. and R. C. Moellering Jr. 1995. Antibacterial agents p. 1281-1307. In P. R. Murray E. J. Baron M. A. Pfaller F. C. Tenover and R. H. Yolken (ed.) Manual of clinical microbiology 6th ed. ASM Press Washington D.C."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.10.2504"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.46.10.3261-3264.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.46.10.3261-3264.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T17:19:48Z","timestamp":1645463988000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.46.10.3261-3264.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,10]]},"references-count":23,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2002,10]]}},"alternative-id":["10.1128\/AAC.46.10.3261-3264.2002"],"URL":"https:\/\/doi.org\/10.1128\/aac.46.10.3261-3264.2002","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,10]]},"assertion":[{"value":"2001-10-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-07-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}