{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:33:32Z","timestamp":1790375612785,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The multiresistance plasmid pJHCMW1, harbored by a clinical\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            strain isolated from a neonate with meningitis, was sequenced. A circular sequence of 11,354 bp was generated, of which 7,993 bp make up Tn\n            <jats:italic>1331<\/jats:italic>\n            , a transposon including the antibiotic resistance genes\n            <jats:italic>aac<\/jats:italic>\n            (\n            <jats:italic>6<\/jats:italic>\n            \u2032)\n            <jats:italic>-Ib<\/jats:italic>\n            ,\n            <jats:italic>aadA1<\/jats:italic>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-9<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-1<\/jats:sub>\n            . The gene\n            <jats:italic>aac<\/jats:italic>\n            (\n            <jats:italic>6<\/jats:italic>\n            \u2032)\n            <jats:italic>-Ib<\/jats:italic>\n            is included in a gene cassette, and both\n            <jats:italic>aadA1<\/jats:italic>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-9<\/jats:sub>\n            are included in a single-gene cassette that may have arisen as a consequence of a recombination event involving two integrons. The pJHCMW1 plasmid replicates through a ColE1-like RNA-regulated mechanism, includes a functional\n            <jats:italic>oriT<\/jats:italic>\n            , and two loci with similarity to XerCD site-specific recombination target sites involved in plasmid stabilization by the resolution of multimers. One of these two loci,\n            <jats:italic>mwr<\/jats:italic>\n            , is active and has been the subject of previous studies, and the other,\n            <jats:italic>dxs<\/jats:italic>\n            , is not functional but binds the recombinase XerD with low affinity. Two additional open reading frames were identified, one with low similarity to two hypothetical membrane proteins from\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            and\n            <jats:italic>Mycobacterium leprae<\/jats:italic>\n            and the other with low similarity to\n            <jats:italic>psiB<\/jats:italic>\n            , a gene encoding a function that facilitates the establishment of the transferring plasmid in the recipient bacterial cell during the process of conjugation.\n          <\/jats:p>","DOI":"10.1128\/aac.46.11.3422-3427.2002","type":"journal-article","created":{"date-parts":[[2002,10,16]],"date-time":"2002-10-16T23:01:16Z","timestamp":1034809276000},"page":"3422-3427","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":71,"title":["Complete Nucleotide Sequence of\n            <i>Klebsiella pneumoniae<\/i>\n            Multiresistance Plasmid pJHCMW1"],"prefix":"10.1128","volume":"46","author":[{"given":"Renee","family":"Sarno","sequence":"first","affiliation":[{"name":"Department of Biological Science, Institute of Molecular Biology and Nutrition, College of Natural Science and Mathematics, California State University Fullerton, Fullerton, California 92834-6850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glen","family":"McGillivary","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Miami University, Oxford, Ohio 45056"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Sherratt","sequence":"additional","affiliation":[{"name":"Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis A.","family":"Actis","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Miami University, Oxford, Ohio 45056"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo E.","family":"Tolmasky","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Institute of Molecular Biology and Nutrition, College of Natural Science and Mathematics, California State University Fullerton, Fullerton, California 92834-6850"},{"name":"Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Barre F. and D. 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