{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:09:01Z","timestamp":1793419741235,"version":"4.5.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 \u03bcg\/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient,\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            ATCC 19424, by transformation of the penicillin-binding protein 2 gene (\n            <jats:italic>penA<\/jats:italic>\n            ) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 \u03bcg\/ml). The sequences of\n            <jats:italic>penA<\/jats:italic>\n            in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported\n            <jats:italic>N. gonorrhoeae penA<\/jats:italic>\n            gene sequences. Some regions in the transpeptidase-encoding domain in this\n            <jats:italic>penA<\/jats:italic>\n            gene were similar to those in the\n            <jats:italic>penA<\/jats:italic>\n            genes of\n            <jats:italic>Neisseria perflava<\/jats:italic>\n            (\n            <jats:italic>N. sicca<\/jats:italic>\n            ),\n            <jats:italic>Neisseria cinerea<\/jats:italic>\n            ,\n            <jats:italic>Neisseria flavescens<\/jats:italic>\n            , and\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            . These results showed that a mosaic-like structure in the\n            <jats:italic>penA<\/jats:italic>\n            gene conferred reductions in the levels of susceptibility of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            to cephems and penicillin in a manner similar to that found for\n            <jats:italic>N. meningitidis<\/jats:italic>\n            and\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.46.12.3744-3749.2002","type":"journal-article","created":{"date-parts":[[2002,11,15]],"date-time":"2002-11-15T20:02:26Z","timestamp":1037390546000},"page":"3744-3749","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":206,"title":["Mosaic-Like Structure of Penicillin-Binding Protein 2 Gene (\n            <i>penA<\/i>\n            ) in Clinical Isolates of\n            <i>Neisseria gonorrhoeae<\/i>\n            with Reduced Susceptibility to Cefixime"],"prefix":"10.1128","volume":"46","author":[{"given":"Satoshi","family":"Ameyama","sequence":"first","affiliation":[{"name":"Research Laboratories, Toyama Chemical Co., Ltd., 2-4-1, Shimookui, Toyama, 930-8508"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoichi","family":"Onodera","sequence":"additional","affiliation":[{"name":"Department of Urology, Jikei University, School of Medicine, 3-19-18, Nishishinbashi, Minato-ku, Tokyo, 105-8741"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Takahata","sequence":"additional","affiliation":[{"name":"Research Laboratories, Toyama Chemical Co., Ltd., 2-4-1, Shimookui, Toyama, 930-8508"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinzaburo","family":"Minami","sequence":"additional","affiliation":[{"name":"Research Laboratories, Toyama Chemical Co., Ltd., 2-4-1, Shimookui, Toyama, 930-8508"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuko","family":"Maki","sequence":"additional","affiliation":[{"name":"Medical Information Center, Toyama Chemical Co., Ltd., 3-2-5, Nishishinjuku, Shinjuku-ku, Tokyo 160-0023"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhisa","family":"Endo","sequence":"additional","affiliation":[{"name":"Department of Urology, JR Tokyo General Hospital, 2-1-3, Yoyogi, Shibuya-ku, Tokyo, 151-8528"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Goto","sequence":"additional","affiliation":[{"name":"Department of Urology, Jikei University, School of Medicine, 3-19-18, Nishishinbashi, Minato-ku, Tokyo, 105-8741"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroo","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Urology, Kosei Hospital, 5-25-15, Yayoi-cho, Nakano-ku, Tokyo, 164-8617, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiko","family":"Oishi","sequence":"additional","affiliation":[{"name":"Department of Urology, Jikei University, School of Medicine, 3-19-18, Nishishinbashi, Minato-ku, Tokyo, 105-8741"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s101560170034"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.3.285"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01297.x"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.2.333-337.1994"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1990.tb00664.x"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.4.1020"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.18.5.730"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00101.x"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.6.856"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.11.2799"},{"key":"e_1_3_1_12_2","first-page":"40","volume":"10","year":"1999","unstructured":"Kumamoto, Y., T. 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