{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:35:38Z","timestamp":1762864538611},"reference-count":16,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-92<\/jats:sub>\n            gene was cloned from a\n            <jats:italic>Proteus mirabilis<\/jats:italic>\n            isolate and expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . Production of the enzyme caused reduction of susceptibility to penicillins and narrow- to expanded-spectrum cephalosporins but not to moxalactam and cephamycins. Determination of kinetic parameters with the purified enzyme revealed hydrolysis of expanded-spectrum cephalosporins, while cephamycins, moxalactam, and aztreonam were very poorly or not hydrolyzed. Clavulanate and penicillanic acid sulfones acylated TEM-92 slowly, and deacylation occurred at measurable rates.\n          <\/jats:p>","DOI":"10.1128\/aac.46.12.3981-3983.2002","type":"journal-article","created":{"date-parts":[[2002,11,15]],"date-time":"2002-11-15T20:02:26Z","timestamp":1037390546000},"page":"3981-3983","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Biochemical Characterization of TEM-92 Extended-Spectrum \u03b2-Lactamase, a Protein Differing from TEM-52 in the Signal Peptide"],"prefix":"10.1128","volume":"46","author":[{"given":"Mariagrazia","family":"Perilli","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche, Universita\u0300 di L'Aquila, I-67100, L'Aquila"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardetta","family":"Segatore","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche, Universita\u0300 di L'Aquila, I-67100, L'Aquila"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Rosaria","family":"De Massis","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche, Universita\u0300 di L'Aquila, I-67100, L'Aquila"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Pagani","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Morfologiche, Eidologiche e Cliniche, Sezione di Microbiologia, Universita\u0300 di Pavia, I-27100, Pavia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Luzzaro","sequence":"additional","affiliation":[{"name":"Ospedale di Circolo di Varese, I-21100, Varese"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, I-53100, Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianfranco","family":"Amicosante","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche, Universita\u0300 di L'Aquila, I-67100, L'Aquila"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/38.1.127"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.4.1278-1280.2001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(87)90609-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"3438","DOI":"10.1073\/pnas.79.11.3438","volume":"79","year":"1982","unstructured":"Inouye, S., X. 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