{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:11:13Z","timestamp":1791187873840,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2002,3,1]],"date-time":"2002-03-01T00:00:00Z","timestamp":1014940800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            As seen by the disk diffusion method, the clinical strain of\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            Pa695, resistant to all extended-spectrum cephalosporins and aminoglycosides, exhibited an unusual synergistic effect between ceftazidime and imipenem. This isolate produced an extended-spectrum \u03b2-lactamase (ESBL) with a pI of 5.8 that appeared to be chromosomally encoded. Cloning experiments revealed that this ESBL was encoded by\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-1<\/jats:sub>\n            , previously described in an integron from\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            . In\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            Pa695, a higher level of resistance to ceftazidime than to ticarcillin was observed, and no synergy between the \u03b2-lactamase inhibitors and extended-spectrum cephalosporins was detected, in contrast to the resistance pattern observed in\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            . Further sequence analysis demonstrated that the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GES-1<\/jats:sub>\n            gene cassette was located in a class 1 integron, which contained another sequence corresponding to the fused\n            <jats:italic>aac(3)-Ib<\/jats:italic>\n            and\n            <jats:italic>aac(6<\/jats:italic>\n            \"\n            <jats:italic>)-Ib<\/jats:italic>\n            \" gene cassettes. The fusion product was functional, as was the product of each gene cloned separately: AAC(3)-I, despite the deletion of the four last amino acids, and AAC(6\"), which carried three amino acid changes compared with the most homologous sequence. The AAC(3)-I protein conferred an expected gentamicin and fortimicin resistance, and the AAC(6\"), despite the Leu-119\u2192Ser substitution, yielded resistance to kanamycin, tobramycin, and dibekacin, but slightly affected netilmicin and amikacin, and had no apparent effect on gentamicin. The fusion product conveyed a large profile of resistance, combining the AAC(6\") activity with a higher level of gentamicin resistance without accompanying fortimicin resistance.\n          <\/jats:p>","DOI":"10.1128\/aac.46.3.638-645.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"638-645","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":113,"title":["Molecular Characterization of a Novel Class 1 Integron Containing\n            <i>bla<\/i>\n            <sub>GES-1<\/sub>\n            and a Fused Product of\n            <i>aac(3)-Ib\/aac(6<\/i>\n            \"\n            <i>)-Ib<\/i>\n            \" Gene Cassettes in\n            <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"46","author":[{"given":"Ve\u0301ronique","family":"Dubois","sequence":"first","affiliation":[{"name":"Laboratoire de Microbiologie, Faculte\u0301 de Pharmacie, Universite\u0301 de Bordeaux 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Marie","sequence":"additional","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie, Ho\u0302pital Pellegrin, Bordeaux"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Arpin","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie, Faculte\u0301 de Pharmacie, Universite\u0301 de Bordeaux 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudine","family":"Quentin","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie, Faculte\u0301 de Pharmacie, Universite\u0301 de Bordeaux 2"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.12.2960"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.3.686"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.2.209"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-43-4-300"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01667.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.5.1574-1585.1992"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.167.2.631-638.1986"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.7.1456"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.9.2247-2253.2000"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.1.175-182.2001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.20.6286-6294.1994"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02368.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.145.3.1365-1373.1981"},{"key":"e_1_3_2_17_2","first-page":"517","year":"1999","unstructured":"Kiska, D. 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