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Treatment of tumor cells with chemotherapeutic agents such as anthracyclines, epipodophyllotoxins, and\n            <jats:italic>Vinca<\/jats:italic>\n            alkaloids results in induction of P-gp expression. This study was performed to determine if clinically relevant antimicrobial drugs (i.e., drugs that are used to treat bacterial infections in cancer patients) other than antineoplastic agents can induce expression of P-gp in MCF-7 breast carcinoma cells. Expression of P-gp and\n            <jats:italic>MDR1<\/jats:italic>\n            mRNA was determined in samples from MCF-7 cells that were treated in culture with doxorubicin (positive control) and the antimicrobial drugs doxycycline, piperacillin, and cefoperazone. The functional status of P-gp was assessed using laser cytometry to determine intracellular doxorubicin concentrations. The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay was used to determine if the cytotoxicity of experimental drugs was related to their ability to induce P-gp expression. MCF-7 cells treated with doxycycline (MCF-7\/doxy) were stimulated to overexpress P-gp, whereas cells treated with piperacillin and cefoperazone did not overexpress P-gp. MCF-7\/doxy cells were compared to a positive-control subline, MCF-7\/Adr, previously selected for doxorubicin resistance, and to MCF-7 cells treated with doxorubicin (MCF-7\/doxo). All three sublines overexpressed P-gp and\n            <jats:italic>MDR1<\/jats:italic>\n            mRNA and accumulated less intracellular doxorubicin than did control MCF-7 cells. P-gp expression was induced only by experimental drugs that were cytotoxic (doxorubicin and doxycycline). Doxycycline, a drug that has been used for treatment of bacterial infections in cancer patients, can induce functional P-gp expression in cancer cells, resulting in multidrug resistance.\n          <\/jats:p>","DOI":"10.1128\/aac.46.3.755-761.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"755-761","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Doxycycline Induces Expression of P Glycoprotein in MCF-7 Breast Carcinoma Cells"],"prefix":"10.1128","volume":"46","author":[{"given":"Katrina L.","family":"Mealey","sequence":"first","affiliation":[{"name":"Department of Veterinary Physiology and Pharmacology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rola","family":"Barhoumi","sequence":"additional","affiliation":[{"name":"Veterinary Anatomy and Public Health, Texas A&amp;M University College of Veterinary Medicine, College Station, Texas 77843-4466"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert C.","family":"Burghardt","sequence":"additional","affiliation":[{"name":"Veterinary Anatomy and Public Health, Texas A&amp;M University College of Veterinary Medicine, College Station, Texas 77843-4466"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Safe","sequence":"additional","affiliation":[{"name":"Department of Veterinary Physiology and Pharmacology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah T.","family":"Kochevar","sequence":"additional","affiliation":[{"name":"Department of Veterinary Physiology and Pharmacology"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.1056\/NEJM199511233332103","volume":"333","year":"1995","unstructured":"Baldini, N., K. 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